Re: [ccp4bb] Co-Crystallization with drug molecule

Dear Fritz, You are right. The recollection of that striking 2015 paper was somehow too strong to be 100% faithful: these are two different modes of using the ligand deposited on a solid support by drying a solution of it, to avoid the DMSO problem. Best wishes, Gerard. -- On

Re: [ccp4bb] Co-Crystallization with drug molecule

:) Gerard, "soaking"! Thanks for the reference! cheers guenter Dear Guenter, The first description of the idea behind this technique that I am aware of is that published almost 10 years ago by a French group under the name of "'dry' co-crystallisation" in the following paper:

Re: [ccp4bb] Problem of data reprocessing with XDS

Dear Yimin and CCP4BB readers, The autoPROC Wiki page mentioned in Clemens's message (below) is being regularly updated with the results emerging from extending that comparison to the improved BUILT=20241002 of the 20240630 version of XDS announced in Kay's message. With best wishes,

Re: [ccp4bb] Co-Crystallization with drug molecule

Dear Guenter, The first description of the idea behind this technique that I am aware of is that published almost 10 years ago by a French group under the name of "'dry' co-crystallisation" in the following paper: http://dx.doi.org/10.1107/S1399004715010342 Best wishe

Re: [ccp4bb] Co-Crystallization with drug molecule

Hi Amit, like Artem wrote below, even despite poor solubility, soaks with dried compounds work well (also in our hands). We adapted it from the work of T. Barthel, J. Wollenhaupt and Manfred Weiss. Also colleagues from industry report good results. We add compound dissolved in DMSO to the crysta

Re: [ccp4bb] Co-Crystallization with drug molecule

Hi Amit, the questions and suggestions by Eta are important ones and I would like to extend them a little bit. For your apo crystals, instead of adding DMSO in one go, transfer the crystal to a drop with 5% DMSO and wait, if it survives, transfer to 10%, to 20% and finally to 30%. Then, do no

Re: [ccp4bb] Co-Crystallization with drug molecule

Dear Amit, If you will decide to try soakings, you could also try to dehydrate the drop to increase ligand concentration, as well as avoiding to wash the crystals in cryo-protection solutions, as described here (https://journals.iucr.org/j/issues/2022/02/00/ap5042/index.html). Good luck! D

[ccp4bb] Structure Meets Function 35 - ISIDORe

Dear All Register now for the 35th edition of the Instruct-ERIC Structure Meets Function webinar series, taking place 11:00 CET on 15 October 2024. This month's webinar will feature two speakers who have accessed Instruct-ERIC services through ISIDORe. The ISIDORe project aims