uate and professional students.
Interested applicants should send their CV and list of 2-3 references to Jarrod
Mousa, Ph.D. at jarrod.mo...@uga.edu.
Publications of interest are below:
Wen, X., Mousa, J.J., Bates, J. Lamb, R.A., Crowe, J.E., Jardetzky, T.S.
Structural basis for anti
Yes, the hinge region between Fab and Fc is highly flexible. If you look at
IgG by EM you will see many different conformations. Best to use Fab or
scFv.
Jarrod Mousa
On Thu, Jun 22, 2017 at 1:39 PM, Cheng Zhang <chengzh1...@gmail.com> wrote:
> Hi all,
>
> I have a naive
Hi all,
Picture attached.
I solved the structure of a membrane protein using LCP. When trying to
identify the cation-binding site, I co-crystallized with Rb+. I see strong
density corresponding to Rb+, and it was confirmed in an isomorphous
difference map.
One problem: in the native structure I
Hi all,
A little off-topic: I am beginning a post-doc in a non-crystallography lab
in a month, and I am working to get a list together of screens to purchase
before I arrive. Can anyone recommend screens for crystallizing antibodies
(Fab fragments), and Fab-antigen complexes?
Thanks,
-Jarrod
Hi all,
I am wondering what the conditions are when Rfree is less than Rwork. I
have seen this when trying certain models to solve my structure, but I am
not sure why this occurs, or if this is valid or not.
Thanks,
Jarrod Mousa
U. of Florida
had luck
using a 100 uL teflon ferrule (you have to hollow it out a bit) for this
purpose. We have a machine shop here that can do the ferrule removal. I
didn't have much luck doing this on my own.
Hope that helps,
Jarrod Mousa
Graduate Research Assistant
University of Florida
-Jarrod Mousa
On Mon
Hi,
I am trying to solve the structure of a membrane protein. The protein has 12
helices and I have a good molecular replacement model that seems to work for
about half of the structure. I used chainsaw to convert the amino acid residues
to that of my protein sequence, and the density fits the
I am also trying to merge data from multiple crystals that I collected from. I
have tried to reindex the data in CCP4 after indexing in iimosflm, and then put
these .mtz files through sortmtz before scala, but I keep getting an error in
sort mtz:
From ccp4_lwbat: warning: attempt to add new
I can index my data through imosflm with no problems, but when I try to scale
using aimless, the program will not run if I also run truncate (or old
truncate). Here is the error message I get below. Could anyone elaborate on
what this means? Thanks.
P.S. I can take the scaled .mtz file from
