Re: [ccp4bb] where I have been going wrong in crystallization?

2009-12-21 Thread Jeffrey Wilson
, lysozyme crystallized in about 1 hour. Jumping that up to 150mg/ml allowed for crystallization in minutes. Hanging drop behaved similarly. I was using lysozyme from Sigma. Jeff Jeffrey Wilson, Ph.D. University of Cincinnati College of Medicine Molecular Genetics Department 231 Albert Sabin Way

Re: [ccp4bb] temperature after 30 minutes using microscopes ?

2009-01-30 Thread Jeffrey Wilson
Matt's in that the base rarely heats up to any significant degree. Jeff Jeffrey Wilson, Ph.D. University of Cincinnati College of Medicine Molecular Genetics Department 231 Albert Sabin Way MSB 3109A Cincinnati, OH 45267-0524 (513) 558-1360

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-30 Thread Jeffrey Wilson
On Jan 28, 2009, at 3:47 AM, Darren Hart wrote: ps anyone using VNTI might consider a backup of their work by exporting files to .gb format. I don't know if a locked up (expired) version permits this and you will have no notice that it is about to expire. My license recently expired. I h

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-06 Thread Jeffrey Wilson
Ds come to the desktop market, I will probably start using the stereo technology again. That's my 2.1 cents adjusted for inflation. Jeff Jeffrey Wilson, Ph.D. University of Cincinnati College of Medicine Molecular Genetics Department 231 Albert Sabin Way MSB 3109A Cincinnati, OH 45267-0524 (513) 558-4651

[ccp4bb] TLS MAKE_U_POSITIVE special position

2007-02-09 Thread Jeffrey Wilson
ld be very worried about? Should I believe the output R and Rfree? Jeffrey Wilson, Ph.D. University of Cincinnati College of Medicine Molecular Genetics Department 231 Albert Sabin Way MSB 3109A Cincinnati, OH 45267-0524 (513) 558-4651