Our group has an opening for a highly motivated postdoctoral fellow, to study
the structure of mammalian ZP domain-containing proteins that mediate egg-sperm
interaction at the beginning of fertilization. Parallel projects on different
ZP domain proteins are also available. For further informati
On Nov 13, 2010, at 11:04 PM, Jerry McCully wrote:
> Dear ALL;
>
> A few weeks ago, I posted my problem with MBP fusion protein.
>
> Thank folks very much for the help. Here is my latest result.
> 1) Shortening the linker region would signifcantly reduce the cleavage of
> target pro
On Sep 8, 2010, at 6:31 AM, Jerry McCully wrote:
> Dear All:
>
> In my case, a 15KD protein without disufide bonds was expressed as
> inclusion bodies in E.coli but can be refolded as monomers with a very low
> solubility.
>
> Adding glycerol did not help so far.
>
> To incre
Apologies for the repetition, but only the first link:
http://cc.oulu.fi/~pkursula/xdsi.html
is for the xdspub program that produces the output I attached. The other one
has the same name, but does something else!
Luca
> WRT the redundancy, I am afraid you have to recompute an approximate value
> yourself using the number of observations and number of unique reflections
> (this is what I do all the time). I suppose one could always write a jiffy
> program to compute the correct values using both files INTEGRA
On Jul 19, 2010, at 12:29 PM, José Ignacio Sánchez Gallego wrote:
> Hi everybody,
> I have to make a 3D structure of my protein, I mutated some aminoacids in
> order to add some N-linked glycans, does anyone know is it possible to show
> the glycosilation on the surface of the 3D structure?? It
Ciao Alessandra,
A couple of pointers:
http://gbio-pbil.ibcp.fr/Curves_plus/Curves+.html
http://rutchem.rutgers.edu/~xiangjun/3DNA/
HTH,
Luca
Luca Jovine, Ph.D.
Group Leader & EMBO Young Investigator
Karolinska I
Dear Tim,
Although it is not routine, it can be done in mammalian cells too! For example,
have a look at this:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.pdf
HTH,
Luca
Luca Jovine, Ph.D.
Karolinska Inst
Dear Claudia,
This could be a good starting point:
Protein Expr Purif. 2008 Apr;58(2):210-21. Epub 2007 Nov 22.
The use of systematic N- and C-terminal deletions to promote production and
structural studies of recombinant proteins.
Gräslund S, Sagemark J, Berglund H, Dahlgren LG, Flores A, Hamma
On 28 Jan 2009, at 16:02, Mark Collins wrote:
Hi
Anybody have suggestions for Mac OsX alternatives?
Thanks in advance,
Mark
Hi Mark,
The latest version of DNA Strider (1.4) runs just fine on both Tiger
and Leopard. For more info, you can contact the author directly at
christian.ma...@cea.
te: 4 April 2008 11:33:56 GMT+02:00
To: Jovine Luca <[EMAIL PROTECTED]>
Cc: CCP4BB@jiscmail.ac.uk
Subject: Re: [ccp4bb] OMIT map from Sfcheck
Please try to use sfcheck binaries or source code from my home page:
http://www.ysbl.york.ac.uk/~alexei/sfcheck.html
Regards
Alexei
Hopefully upda
On 4 Apr 2008, at 01:36, Michael Giffin wrote:
i get the same error on OS X 10.5.2 and 10.4.11:
sfcheck -f refmac2.mtz -m refmac2.pdb -out y -nomit 2 -map
the error:
Open failed: Unit: 11, File: sfcheck_scr.dat (logical:
sfcheck_scr.da
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