Re: [ccp4bb] How to transfer non-frozen crystals with less disturbance?

drop. Hopefully your crystals will survive the transfer (test before). Cheers, Mario Sanches On Wed, Jul 2, 2014 at 1:17 PM, Meisam Nosrati wrote: > Dear CCP4ers > > I need to transfer some crystals mainly in sitting drops to the site of > data collection without freezing them. >

Re: [ccp4bb] 3letter code

> > Faisal > School of Life Sciences > JNU > -- Mario Sanches http://ca.linkedin.com/in/mariosanches

Re: [ccp4bb] Unidentified blobs

lobs shown in the structure models? > > Attached please find the images of the two blobs. Any clue what it is? > > Thank you for your comments and advices > > Uma > > > -- Mario Sanches http://ca.linkedin.com/in/mariosanches

Re: [ccp4bb] Superpositions: Deviation by Residue

Hi Jacob, I used Moleman to do that once (http://xray.bmc.uu.se/usf/moleman2_man.html ) The Distance LIst command is probably what you are looking for. Mario Sanches On Mon, Nov 28, 2011 at 5:22 PM, Jacob Keller < j-kell...@fsm.northwestern.edu> wrote: > Dear Crystallographer

Re: [ccp4bb] Ramachandran plot difference between Coot and Morprobity (or Phenix)

validation. Good luck, Mario Sanches 2011/9/30 Xiaopeng Hu > Dear all, > > I just notified that there is a big difference between the Ramachandran > plot analysis results produced by Coot and Morprobity (or Phenix). For the > structure I am working now, Phenix(Morprobity) gives o

Re: [ccp4bb] Protein elution in Size Exclusion

Testing for cracks in the column is quite easy. Take whichever standard you happen to have (lisozyme, GST, etc) and do a run. If it comes out in a single peak the problem is with your prep. If it comes out throughout the run the problem is with your column. Mario Sanches On Sun, Aug 28, 2011 at

Re: [ccp4bb] S-200 buffer-based peak shift?

ist Training Program > cel: 773.608.9185 > email: j-kell...@northwestern.edu > *** > -- Mario Sanches Postdoctoral Fellow Samuel Lunenfeld Research Institute Mount Sinai Hospital 600 University Ave Toronto - Ontario Canada M5G 1X5 http://ca.linkedin.com/in/mariosanches

Re: [ccp4bb] Synthetic RNA for Crystallization

are many good references out there that deal with production, purification and crystallization or RNA. Good luck, Mario Sanches On Sun, Mar 13, 2011 at 4:39 AM, Michael Thompson wrote: > Hello All, > > I am looking for some advice from some experienced RNA crystallographers. I > woul

Re: [ccp4bb] coot can't read my mtz and pdb file

put mtz as the input for coot. The Refmac mtz will have the map coefficient columns on it. Good luck, Mario Sanches 2011/3/7 王瑞 > I have an ambiguous question that can't decide where it belongs . In fact, > I was going to refine my data after molecular replacement, but coot can't

Re: [ccp4bb] disulfide bridge in red density

Hi Sadaf, You may want to try to play with the occupancy. I have had a case before where the disulfide bond had double occupancy. In my case it was obvious because I could see the other density, but sometimes you can just see a major one due to disorder. I hope that helps, Mario Sanches On Sun

Re: [ccp4bb] Low number of waters for a given resolution

D<http://www.ncbi.nlm.nih.gov/pubmed?term=%22Bordo%20D%22%5BAuthor%5D> . Mario Sanches On Wed, Feb 16, 2011 at 3:48 PM, Filip Van Petegem < filip.vanpete...@gmail.com> wrote: > Hello all, > > Some crystal structures seem to contain a very low number of water > molecules given their resolut

Re: [ccp4bb] protein interaction

ive gel analysis (or other assays using purified protein) but > protein complex crystal structure was solved. > > > > Thanks > > Happy Thanksgiving > > Yogi > > > > > -- Mario Sanches Postdoctoral Fellow Samuel Lunenfeld Research Institute Mount Sinai Hospital 600 University Ave Toronto - Ontario Canada M5G 1X5 http://ca.linkedin.com/in/mariosanches

Re: [ccp4bb] problem installing arp/warp

figured in /etc/bash.bashrc so that they will be visible everywhere for all users, so I think that was not the issue here. Thank you again for the quick reply, Mario Sanches Gerrit Langer wrote: Dear Mario, it seems that inside the install script the path variable is not set and therefore the

[ccp4bb] problem installing arp/warp

Dear all, I am trying to install arp/warp and I am stuck with the following error: -- Checking refmac5 installation - refmac5: Command not found. *** ERROR *** Cannot execute refmac5 *

Re: [ccp4bb] unknown symmetric density

Same thing to me. I was able to assign a PEG molecule to a similar density. Mario Sanches Jiamu Du wrote: Oh.. Thank you. I will try to fit it with a piece of PEG. Best wishes. On Tue, Feb 26, 2008 at 10:38 PM, <[EMAIL PROTECTED] <mailto:[EMAIL PROTECTED]>> wrote:

[ccp4bb] SUMMARY: Sealing deep wells

cold seals? I would appreciate if people could tell me which seal they use, and at which temperature. Thank you all in advance, Mario Sanches ## Those are the answers that I received: We did extensive testing for this using caps, mat caps, tape, adhesive foil, heat seal plastic

[ccp4bb] sealing deep wells

all in advance, -- Mario Sanches Laboratório Nacional de Luz Síncrotron Cristalografia e Espectroscopia de Biomoléculas Tel: +55 19 3512 1275

Re: [ccp4bb] Multiple nucleation

is, E., Dierks, K., Moreno, A., Dieckmann, W.M., and Chayen, N.E. (2002) Separating nucleation and growth in protein crystallization using dynamic light scattering, Acta Cryst. Sect. D, 58, 1597-1600. HTH, Mario Sanches Jobichen Chacko wrote: Hai All, Sorry for the non Ccp4 question. I got