Re: [ccp4bb] PyMol and Schrodinger

I'd like to add the under-underdog then, Tim Fenn's povscript+ https://sites.google.com/site/timfenn/povscript. Makes really clear and nice figures and is rather simple to compile. For the most part you do not even need a mouse to use it ... Michael On 23.04.2014 18:21, mark.x.brooks wrote:

Re: [ccp4bb] crystallizing fusion proteins

For us, a C-terminal macro domain tag worked well to crystallize a rather flexible target protein. https://pubmed.ncbi.nlm.nih.gov/27774698/ best regards, Michael On 15.03.21 16:51, Pascal Egea wrote: Hi Herman, I will add a few points to all the excellent advice already provided to you. If

[ccp4bb] Linux stereo set-up

Dear all, sorry for the off topic question (that has been asked many times before, but the hardware keeps changing): I want to buy a linux workstation with Nvidia 3D vision and an Alienware OptX AW2310 23-inch 3D TFT monitor. This set-up suggested by the CCP4 wiki for TFT stereo. Has anyone a

Re: [ccp4bb] Off Topic: Isothermal Titration Calorimetry (ITC)

Dear Jose, I used both instruments for a number of years, first the VP ITC and later on the ITC 200. I personally find the ITC 200 much more demanding to operate. I think Microcal managed to improve the sensitivity of the ITC 200 about 2-3 fold compared to the VP ITC, but they decided to decr

[ccp4bb] Coot binary for Ubuntu 10.04 64 bit

Hi, can someone point me to a recent coot binary that runs under Ubuntu 10.04 64 bit? There are so many compiling issues that I am about to give up compiling it myself thanks! Michael

Re: [ccp4bb] Coot binary for Ubuntu 10.04 64 bit

On 12/07/10 7:28 AM, Michael Hothorn wrote: Hi, can someone point me to a recent coot binary that runs under Ubuntu 10.04 64 bit? There are so many compiling issues that I am about to give up compiling it myself thanks! Michael

[ccp4bb] error during mrbump installation

Hi, I am trying to install mrbump on a 32 bit ubuntu linux desktop (kernel: 2.6.31-22-generic, python 2.4, 2.5 and 2.6 tested). When executing "python setup.py" after extracting mrbump-0.4.4, I get the following error messages: setup.py:269: DeprecationWarning: os.popen4 is deprecated. Use

[ccp4bb] small crystallisation incubator

Dear all, sorry for the off-topic question. I am looking for an affordable+small crystallisation incubator (16-21 deg Celsius). Ideally, it would fit neatly under my bench (in a genetics lab) and hold a few dozen 96 and some 24 well plates. A friend already suggested to get a wine-cooler?! I

Re: [ccp4bb] crystallization incubators

Dear Alexandra, I bought this one recently and it is very nice. Very low vibration and enough space for about 25 24-well plates plus maybe 50 96-well plates. The only problem I had is that in my case the temperature reading on the external LCD display is slightly off compared to the 'real' te

[ccp4bb] ultrafiltration/buffer exchange device for insect cell cultures

Dear all, sorry for the non-CCP4 question. I am expressing a protein in insect cells that is targeted for secretion into the medium. After expression, I want to concentrate the medium containing my protein of interest (from 10 l to 0.3 L) and at the same time change the buffer to something tha

Re: [ccp4bb] Se oxidation

Hi, I wouldn't bother either. I once phased a structure based on about 160 oxidized Se in the AU (Hothorn et al., JBC, 2006). Just make sure that most of them are oxidized and do a proper absorption scan. best Michael aka akaka wrote: Dear All I would like to know whether oxidation of Se

[ccp4bb] ERC-funded postdoc positions at the University of Geneva, Switzerland

<http://structplantbio.org/> and https://bit.ly/2Pqq3Or Thank you & best wishes, Michael -- Michael Hothorn Associate Professor & EMBO Young Investigator HHMI International Research Scholar Structural Plant Biology Laboratory Department of Botany and Plant Biology Sciences III

[ccp4bb] Peristaltic pump for affinity chromatography

Dear all, sorry for the off-topic question. I want to buy a simple peristaltic pump to apply larger sample volumes (300-500 mL) to simple affinity columns (desired flow-rates: 2-8 mL/min). I was wondering whether the (good?)-old Pharmacia/GE P1 pump still represents the best system engineers have

[ccp4bb] PhD studentship at University of Geneva, Switzerland; Structural and biochemical analysis of inositol pyrophosphate signaling pathways

Applications are invited for a PhD position, starting any time in 2019, in the group Prof. Michael Hothorn at University of Geneva (http://structplantbio.org). The lab combines structural biology (X-ray protein crystallography, cryo electron microscopy) and quantitative biochemistry with

Re: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] AW: [ccp4bb] challenges in structural biology

Dear Patrick, plants do have a rather complex immune system. best regards, Michael On September 21, 2019 10:18:39 AM GMT+02:00, Patrick Shaw Stewart wrote: >Dear Herman > >Animals that are sick tend not to move around a lot. One can imagine >that >this limits the tendency for animal viruses an