Dear Patrick, plants do have a rather complex immune system. best regards, Michael
On September 21, 2019 10:18:39 AM GMT+02:00, Patrick Shaw Stewart <patr...@douglas.co.uk> wrote: >Dear Herman > >Animals that are sick tend not to move around a lot. One can imagine >that >this limits the tendency for animal viruses and other animal pathogens >to >become more and more virulent, because the very virulent strains won't >spread as fast. And (importantly) when the most virulent strains >finally >arrive at some particular location, they will find that their potential >hosts are already immune*. > >Since plants don't move around, I have always wondered why plant >pathogens >don't increase in virulence until they wipe out their hosts, especially >when you bear in mind that plants don't have complex immune systems. > >Could these multiple genes be a way to avoid being wiped out by >disease? >Ie if the plant gets sick, it just switches on a batch of "reserve" >genes**. Is that possible? > >Thx, Patrick > > >* This is a pet theory of mine: https://oldwivesandvirologists.blog > >**Or maybe the expression of these genes is random - two genetically >identical individuals growing side-by-side might express different >batches >of genes on a random basis. Again, this might be mainly about disease >prevention. > > > >On Fri, Sep 20, 2019 at 8:51 AM <herman.schreu...@sanofi.com> wrote: > >> Dear John, >> >> Plants cannot walk away to a more favorable spot. They remain stuck >where >> they germinate, e.g. whether the place is sunny, shady, wet, dry, >fertile, >> poor etc. So plants compensate by having a lot of genes available to >be >> able to adapt to the particular spot where happen to be. And indeed, >plants >> have usually more genes then animals! >> >> Best, >> >> Herman >> >> >> >> *Von:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *Im Auftrag >von >> *John R Helliwell >> *Gesendet:* Freitag, 20. September 2019 09:19 >> *An:* CCP4BB@JISCMAIL.AC.UK >> *Betreff:* [EXTERNAL] Re: [ccp4bb] AW: [ccp4bb] challenges in >structural >> biology >> >> >> >> *EXTERNAL : *Real sender is owner-ccp...@jiscmail.ac.uk >> >> >> >> Dear Martin, >> >> Many thanks for these details of the size of the human genome over >the >> decades and also the news of your most interesting upcoming review. I >shall >> read it with great interest. >> >> Incidentally is the over 40000 genes for the rice genome number >correct? >> This number caught my eye as being interesting how the rice genome is >more >> complicated than our genome. >> >> Best wishes, >> >> John >> >> Emeritus Professor John R Helliwell DSc >> >> >> >> >> >> >> >> >> On 19 Sep 2019, at 08:35, Kollmar, Martin <m...@nmr.mpibpc.mpg.de> >wrote: >> >> Dear John, >> >> the „100,000 human genes“ is a long-standing myth broad forward by >the >> initiators of the U.S. human genome sequencing projects in 1990. This >large >> number completely contradicted all genetics and mutation data since >the 1940 >> th, but the sequencing community (genome, cDNA, EST) didn’t read even >the >> standard text books. Thus, the “30,000” genes published with the two >human >> genome papers in 2001 are not “surprisingly low” but just in >accordance >> with the predictions and the data since the 1940th. The gene number >went >> down to about 23,000 already in 2004, and the current numbers >(depending on >> database) range around 20,000 human protein-coding genes. The myth of >the >> large numbers is only propagated by those who profit from larger >numbers >> (e.g. bigger grants, papers in higher IF journals, big consortia). >> >> >> >> I have written a review about the current state (and history) of the >human >> protein-coding genes, which will appear online in BioEssays soon and >> finally in the November issue (will be open access). In this review >there >> will be some (hopefully) useful plots showing the gene numbers since >the >> 1940th and a detailed review of all the numbers and their >experimental >> basis (most were actually just extrapolations from small-scale data). >> >> >> >> Please excuse this kind of self-advertisement, but it is really more >than >> time to move this myth out of science literature and communication. >> >> >> >> Best regards, >> >> Martin >> >> >> >> Priv. Doz. Dr. Martin Kollmar >> >> >> >> Group Systems Biology of Motor Proteins >> >> Department NMR-based Structural Biology >> >> Max-Planck-Institute for Biophysical Chemistry >> >> Am Fassberg 11 >> >> 37077 Goettingen >> >> Deutschland >> >> >> >> www.motorprotein.de >> ><https://urldefense.proofpoint.com/v2/url?u=http-3A__www.motorprotein.de_&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=IWuNyBzheAGJ761iddc78L4lz7sB21cKQTrawbV4j0M&e=> >> (Homepage) >> >> www.cymobase.org >> ><https://urldefense.proofpoint.com/v2/url?u=http-3A__www.cymobase.org_&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=fMYqMfsorSLNspHq5-Wx_WDkChCYsDr9NEXwrGsKvpo&e=> >> (Database of Cytoskeletal and Motor Proteins) >> >> www.diark.org >> ><https://urldefense.proofpoint.com/v2/url?u=http-3A__www.diark.org_&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=QB0-HJUz9wLSC-Xd5_Bj6Gnhx70AaYoxkD8kIu7Ub6A&e=> >> (diArk - a resource for eukaryotic genome research) >> >> www.webscipio.org >> ><https://urldefense.proofpoint.com/v2/url?u=http-3A__www.webscipio.org_&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=sy5FDRUX22Q-6XcBo-X_-tgZenKZRLf9N__I8x3pnBo&e=> >> (Scipio - eukaryotic gene identification) >> >> >> >> *Von:* CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> *Im Auftrag von >*John >> R Helliwell >> *Gesendet:* Donnerstag, 19. September 2019 08:51 >> *An:* CCP4BB@JISCMAIL.AC.UK >> *Betreff:* Re: [ccp4bb] challenges in structural biology >> >> >> >> Dear James, >> >> Well, 100,000 genes used to be the estimate of the size of the human >> genome. >> >> (eg see >> >https://physicsworld.com/a/protein-crystallography-the-human-genome-in-3-d/ >> ><https://urldefense.proofpoint.com/v2/url?u=https-3A__physicsworld.com_a_protein-2Dcrystallography-2Dthe-2Dhuman-2Dgenome-2Din-2D3-2Dd_&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=Au_C7wioq-2-qa1kL026q6V6n88YzwYRsi2fGz6xjpg&e=> >> ) >> >> It seems it has got easier, albeit still gargantuan, at ~30,000 genes >to >> be expressed into proteins. >> >> >> >> Meanwhile funding agencies also look out for Big Ideas:- >> >> >> >https://epsrc.ukri.org/research/ourportfolio/epsrcbigideas/?utm_source=Twitter&utm_medium=social&utm_campaign=SocialSignIn >> ><https://urldefense.proofpoint.com/v2/url?u=https-3A__epsrc.ukri.org_research_ourportfolio_epsrcbigideas_-3Futm-5Fsource-3DTwitter-26utm-5Fmedium-3Dsocial-26utm-5Fcampaign-3DSocialSignIn&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=TMvBW-JMC-G_OW0woSqqukhb03utq-y672taYl38T9Y&e=> >> >> and even helpfully spell out the difference between a Big Idea and a >Grand >> Challenge! >> >> Maybe an “Open Door for funding” for us all? >> >> >> >> Today also the repertoire of methods capable of resolving in 3D >protein >> structures has expanded further with the splendid development of >cryoEM. >> >> >> >> To define challenges in terms of projects, as Max Perutz taught us >> (“Haemoglobin the Molecular Lung”) avoids methods looking for >problems. >> >> >> >> Also a final thought, how we organise ourselves in different areas of >the >> World varies according to our cultural traditions. So the Big Project >is >> neutral to politics and can accommodate all contributions however so >> arrived at. >> >> >> >> “What shall we do with it?” >> >> As Darwin taught us, first make your Collection...... >> >> >> >> Greetings! >> >> John >> >> >> >> Emeritus Professor John R Helliwell DSc >> >> >> >https://www.crcpress.com/The-Whats-of-a-Scientific-Life/Helliwell/p/book/9780367233020 >> ><https://urldefense.proofpoint.com/v2/url?u=https-3A__www.crcpress.com_The-2DWhats-2Dof-2Da-2DScientific-2DLife_Helliwell_p_book_9780367233020&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=xJvbX5e1byn0_V91NpWaz5pd5GEYg9BKtNQKHCkMeKM&e=> >> >> >> >> >> >> >> On 18 Sep 2019, at 22:15, James Holton <jmhol...@lbl.gov> wrote: >> >> Thank you John, an excellent choice as always. Here is your trillion >> dollars! Now, what are you going to do with it? >> >> Do you think simply scaling up current technology could reach this >goal? >> More screens, more combinations, more compute cycles? Remember, if >you >> want the "genome/proteome" you need all of it, including all those >> super-cool human membrane proteins we gave up on because they were >too >> hard. >> >> I think we all have at least one of those projects in our past. What >was >> the show-stopper in the end? Did they just not grow crystals? Poor >> diffraction? Weird diffraction? Twinned? Won't phase? Won't refine to >a >> decent R factor? Annoying reviewer? Did you try cryoEM? NMR? and did >they >> not work either? >> >> I think a key question for all of us is: what new capability would >make >> you decide to go back and pick up your old favorite project again? >Without >> your structure, the genome is incomplete. >> >> -James Holton >> MAD Scientist >> >> On 9/16/2019 12:24 AM, John R Helliwell wrote: >> >> Dear James, >> >> Here you go, a “grand challenge” suggestion to consider for funding >from >> the “James Holton Foundation for structural biology research”:- >> >> “The human genome/proteome in 3-D” >> >> Greetings, >> >> John >> >> Emeritus Professor John R Helliwell DSc >> >> >> >> >> >> >> >> >> On 14 Sep 2019, at 02:39, James Holton <jmhol...@lbl.gov> wrote: >> >> >> I would like to thank everyone who took the time to respond to my >question >> that started this thread. It is really good for me to get a sense of >the >> community perspective. Some debates were predictable, others not. >Many >> ideas I agree with, some not so much. All were thought-provoking. I >think >> this is going to be a really good GRC! >> >> Something I did not expect to distill from all the responses is that >the >> dominant challenge in structural biology is financial. The most >common >> strategy suggested for addressing this challenge was torpedoing other >> scientists in similar fields, perhaps expecting to benefit from the >> flotsam. Historically, this strategy is often counterproductive and >at >> best inefficient. The good news is there is a lot of room for >improvement. >> In reality, we are all on the same ship, and the people in our >funding >> agencies fighting to get us what we need can be much more effective >when >> armed with positive ideas and clear plans. That is a better strategy >for >> overcoming this challenge. >> >> To this end, my first GRC session title is going to be: >> >> "If I had a trillion dollars for structural biology" >> >> I think we can all agree that science in general is vastly >under-funded >> relative to the impact it has on the human condition. For example, I >> estimate the value of a general cure for cancer to be at least a >trillion >> dollars. This is based on the lives claimed every year, multiplied >by how >> much one person would gladly pay after being diagnosed (amortized >over the >> rest of their much longer life). This is only ~1% of the Gross World >> Product, a real bargain if we can come up with a plan that will >actually >> work. >> >> Now, obviously not all cancer research is structural biology, but not >all >> structural biology is cancer research either. Let us suppose for a >moment >> that you (yes, I'm talking to YOU), were given a trillion-dollar >budget to >> do your science. After buying all the tools and hiring all the >people you >> wanted: would that solve all of your problems? I expect not. The >> intellectual and technical challenges that remain are what I believe >> science is really all about, and the 2020 Diffraction Methods GRC >will >> focus on the ones facing structural biology. >> >> My goals here are twofold: >> 1) I believe it would be healthy for this field if we all spent a >little >> time "thinking big" >> 2) I want to remove financial anxiety from the discussion, both here >and >> at the GRC. >> >> I ask for one restraint: please confine the discussion to structural >> biology. I understand it is difficult to think about the >trillion-dollar >> level without involving politics, but the CCP4 Bulletin Board is not >a >> political discussion forum, and neither is the GRC. Assume all the >other >> worthy causes in the world are given their own ample budgets. This >trillion >> is yours, and you have to spend it on structural biology. If you >can't >> think of anything, think harder. >> >> To get you started, a few things that could be done for under a >trillion >> dollars: >> 1) re-do all the protein crystallization in the PDB, 500 times >(saving all >> information) >> 2) buy Google and Facebook, get their AI teams to do machine learning >and >> structure prediction for us >> 3) hire every "biological scientist" in the world, and give each $1M >to >> work on your projects >> 4) re-do the NASA Apollo program three times >> 5) build 1000 XFELs and 100,000 Titan microscopes (yes, that's "and") >> 6) solve the phase problem by brute force. (zettaflops-scale >computing at >> $0.03/gflop) >> 7) build half a dozen terapixel detectors (ask Colin Nave what those >can >> do) >> 8) fund every NIH grant submitted in the last 5 years. Not just the >> awarded ones, all of them. >> 9) X-prize style competitions for landmark achievements, such as >> predicting crystallization outcomes, or finding a universal way to >stop >> protein from denaturing on the air-water interface. >> >> This is not a to-do list, but rather an attempt to convey the scale >of >> what can be done. Oh, and you have a month or so to think about it. >The >> meeting is July 26-31 2020, but my speaker list is due Oct 15. >> >> Now, of course, at the GRC I will not actually have billion-dollar >prizes >> to pass around, but I do want to set our sights on those lofty goals, >and >> then work on the bridge we will need to get there. >> >> So, when I say "challenge" I mean more than something we all agree is >> hard. Those would make for very short talks. I am after something >more >> like a benchmark. Useful challenges should have certain properties. >They >> should be: >> a) possible, because something that doesn't work no matter what you >do is >> no fun. >> b) hard, because something that is too easy is also not very >interesting >> c) realistic, as in relevant to a real-world problem we all agree is >> important >> d) accessible, as in reasonable download sizes and/or affordable >reagents >> e) fast, because it if takes forever to try it nobody will have time >to >> participate >> f) measurable, as in having a clear and broadly acceptable "score" >> g) adjustable, as in the level of "difficulty" can be selected >> continuously between "easy" and "impossible". >> >> This last one is important because it is at the transition point >between >> success and failure that teaches us the most about what can be >improved. >> >> Some challenges that already exist are: >> anomalous phasing from weak signals >> https://bl831.als.lbl.gov/~jamesh/challenge/anom/ >> ><https://urldefense.proofpoint.com/v2/url?u=https-3A__bl831.als.lbl.gov_-7Ejamesh_challenge_anom_&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=0IJQWNeTwpNxj1WNhXurnD-UrpV5RQ1cHpgke0pHcsw&e=> >> anomalous phasing from twinned data >> https://bl831.als.lbl.gov/~jamesh/challenge/twin/ >> ><https://urldefense.proofpoint.com/v2/url?u=https-3A__bl831.als.lbl.gov_-7Ejamesh_challenge_twin_&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=dDs_r6utWD9Sh6VhJST1OsWgNZVKRubPS2-H6PH_Ed0&e=> >> merging highly incomplete data with an indexing ambiguity >> https://bl831.als.lbl.gov/~jamesh/challenge/microfocus/ >> ><https://urldefense.proofpoint.com/v2/url?u=https-3A__bl831.als.lbl.gov_-7Ejamesh_challenge_microfocus_&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=PcG8DXXeww_NLMoXqNEb_esUbiARpm2gZ6daHSSVqMI&e=> >> extracting motions from diffuse scatter data >> https://bl831.als.lbl.gov/~jamesh/challenge/diffuse/ >> ><https://urldefense.proofpoint.com/v2/url?u=https-3A__bl831.als.lbl.gov_-7Ejamesh_challenge_diffuse_&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=Q6TAvbSKkAAxEcS8GMgtDkfFTUAQdm6oiK_xQGRohOw&e=> >> Coming soon: >> dial-a-resolution model building challenge >> XFEL data processing reference set >> >> -James Holton >> MAD Scientist >> >> On 7/25/2019 10:07 AM, Keller, Jacob wrote: >> >> >>It would seem to me that an important issue is also: do get all >> information out of our diffraction data? By integrating the Bragg >peaks we >> usually neglect the diffuse scattering that could potentially contain >> additional (dynamic) structural information. This can be cloudy >diffuse >> scattering hidden in the background but also diffuse streaks that >contain >> information on packing disorder and reveals intrinsic interactions in >the >> crystal. >> >> >> >> Along these lines, and taking a page from you also, how about >> “crystallographic model refinement as image-faking?” Metrics of the >> goodness of a particular refinement could simply be some measure of >the >> correlation between predicted vs. measured images. I have seen some >of this >> done with diffuse scattering, but why not with the whole thing, >including >> intensity and shape of Bragg peaks, solvent rings, etc? Maybe instead >of >> doing the multiple steps of (indexing, integration, scaling, >solving…) all >> of this could be refined as one? Processing parameters like >moscaicity >> [sic] etc would now be part of the final model…? >> >> >> >> JPK >> >> >> >> >> >> >> >> >> Loes Kroon-Batenburg >> >> On 07/15/19 21:44, Holton, James M wrote: >> >> Hello folks, >> >> >> >> I have the distinct honor of chairing the next Gordon Research >> >> Conference on Diffraction Methods in Structural Biology (July 26-31 >> >> 2020). This meeting will focus on the biggest challenges currently >> >> faced by structural biologists, and I mean actual real-world >> >> challenges. As much as possible, these challenges will take the form >of >> >> friendly competitions with defined parameters, data, a scoring >system, >> >> and "winners", to be established along with other unpublished results >> >> only at the meeting, as is tradition at GRCs. >> >> >> >> But what are the principle challenges in biological structure >> >> determination today? I of course have my own ideas, but I feel like >I'm >> >> forgetting something. Obvious choices are: >> >> 1) getting crystals to diffract better >> >> 2) building models into low-resolution maps (after failing at #1) >> >> 3) telling if a ligand is really there or not >> >> 4) the phase problem (dealing with weak signal, twinning and >> >> pseudotranslation) >> >> 5) what does "resolution" really mean? >> >> 6) why are macromolecular R factors so much higher than >small-molecule ones? >> >> 7) what is the best way to process serial crystallography data? >> >> 8) how should one deal with non-isomorphism in multi-crystal methods? >> >> 9) what is the "structure" of something that won't sit still? >> >> >> >> What am I missing? Is industry facing different problems than >> >> academics? Are there specific challenges facing electron-based >> >> techniques? If so, could the combined strength of all the world's >> >> methods developers solve them? I'm interested in hearing the voice >of >> >> this community. On or off-list is fine. >> >> >> >> -James Holton >> >> MAD Scientist >> >> >> >> >> >> >######################################################################## >> >> >> >> To unsubscribe from the CCP4BB list, click the following link: >> >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ><https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFAw&c=LU6cRtx0xgB8s29tIz9Olw&r=eLCg9eJ4Rs_LnxfUWsp7FSxhIEcZYmTSU4Uyq1bRYPI&m=j60Req0Ctc22ORJ8Tr5oxj6jiKrZs6a6a3qScsFKgfg&s=nvFe-NBmWDef18CvLm-0QKC6gd9nDyAZxKO5eEZ7t1I&e=> >> >> >> >> >> >> >> -- >> >> >> >> __________________________________________ >> >> >> >> Dr. Loes Kroon-Batenburg >> >> Dept. of Crystal and Structural Chemistry >> >> Bijvoet Center for Biomolecular Research >> >> Utrecht University >> >> Padualaan 8, 3584 CH Utrecht >> >> The Netherlands >> >> >> >> E-mail : l.m.j.kroon-batenb...@uu.nl >> >> phone : +31-30-2532865 >> >> fax : +31-30-2533940 >> >> __________________________________________ >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> ><https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFAw&c=LU6cRtx0xgB8s29tIz9Olw&r=eLCg9eJ4Rs_LnxfUWsp7FSxhIEcZYmTSU4Uyq1bRYPI&m=j60Req0Ctc22ORJ8Tr5oxj6jiKrZs6a6a3qScsFKgfg&s=nvFe-NBmWDef18CvLm-0QKC6gd9nDyAZxKO5eEZ7t1I&e=> >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> ><https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=9UgmXd4v3TIc9n4xsYN8gLYWH_H3YoFK6twdNr3syaA&e=> >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> ><https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=9UgmXd4v3TIc9n4xsYN8gLYWH_H3YoFK6twdNr3syaA&e=> >> >> >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> ><https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=9UgmXd4v3TIc9n4xsYN8gLYWH_H3YoFK6twdNr3syaA&e=> >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> ><https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=32JRE8HZHPdJxpaoz1sLz-PnTi-D_zZTMfdQs_FdEcI&s=9UgmXd4v3TIc9n4xsYN8gLYWH_H3YoFK6twdNr3syaA&e=> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> > >######################################################################## > >To unsubscribe from the CCP4BB list, click the following link: >https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 -- Sent from my Android device with K-9 Mail. 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