Re: [ccp4bb] iMosflm bug?

2013-06-25 Thread Reginald McNulty
Hi, I have seen this before. I would also check the screen resolution (size) used by the terminal to launch the program matches the largest size capable by your monitor. You can check and change this with the xrandr command. Type man xrandr for info. Cheers, Reginald McNulty On Jun 25, 2013

Re: [ccp4bb] yellow crystals

2011-11-05 Thread REGINALD MCNULTY
avin or protein. Best regards, Reginald McNulty On Nov 5, 2011, at 5:57 PM, Caitlyn Claire Yeykal wrote: > Thanks for all the replies -- there are no suggestions in the literature or > in crystallized or predicted domain structures that this protein binds a > cofactor, and, alt

[ccp4bb] Off-topic: DSF thermo cycler low temp limit

2011-10-26 Thread Reginald McNulty
We are trying to determine Tm value of rather unstable proteins with a Tm in the mid 20 C range using DSF/thermofluor. Our Stratagene thermocycler has a low temp limit of 25 C (Peltier). I called the company and they said it's a 'hardware limit' that cannot be changed. 1) Has anyone been able to

[ccp4bb] APBS

2011-07-05 Thread Reginald McNulty
Dear CCP4BB, I apologize for the off topic question. I have determined the 3D structure of a membrane protein transporter and want to make a figure showing the surface charge. So, I ran PDB2PQR and specified pH 5.4. I understand the next step is to run APBS. I know there are different dielectr

Re: [ccp4bb] Help! low resolution protein-DNA complex

2011-06-16 Thread Reginald McNulty
HENIX (find_helices_strands). This should give the ideal geometry. You can also refine with secondary structure restraints for proper hydrogen bonding patterns. If someone told you to focus on geometry, then I'd focus on the RMS bonds/angles and Ramachandran plot. Best regards, -Reggie -