Re: [ccp4bb] Refolding of Denatured Protein

2009-05-05 Thread Sanjiv Kumar
concentrators. Thanks a lot for help and suggestions. Sanjiv Kumar Lab. No. 411, Functional Genomics Unit, Institute of Genomics and Integrative Biology, New Delhi-110007 India On Tue, May 5, 2009 at 6:40 PM, Shao-Yang Ku wrote: > Your protein concentration may be too high, and the stepwise dilut

Re: [ccp4bb] Refolding of Denatured Protein

2009-05-05 Thread Sanjiv Kumar
aboratory of Molecular Biology > Hills Road, Cambridge. > CB2 0QH > > Tel: 44-1223-402405 > > > > > > -- Sanjiv Kumar Lab. No. 411, Functional Genomics Unit, Institute of Genomics and Integrative Biology, New Delhi-110007 India

Re: [ccp4bb] Refolding of Denatured Protein

2009-05-05 Thread Sanjiv Kumar
Hydrochloride. I can try with both *Guanidine HCl and detergent. Since I got good purification with the 8M urea, I was thinking if this protein could be refolded and used further. Isn't removal of detergent more difficult then Urea? Sanjiv Kumar Lab. No. 411, Functional Genomics Unit, Institu

[ccp4bb] Refolding of Denatured Protein

2009-05-05 Thread Sanjiv Kumar
. Please Help. Sanjiv Kumar