Re: [ccp4bb] R free rising

Are you sure you have identified the correct space group? Cheers Schara Dr. Schara Safarian Project Leader Max Planck Institute of Biophysics Department of Molecular Membrane Biology Max-von-Laue-Straße 3 60438 Frankfurt am Main Deutschland/Germany Visiting Assistant Professor

Re: [ccp4bb] need help with background and over fitting in 2D classification

Hey Suchi, you could firstly increase the binning to factor to 4-5. Also, it would help to shrink the mask radius. Most definitely keep the resolution limited to 8-10 Å! Cheers Schara Dr. Schara Safarian Max Planck Institut für Biophysik Abteilung für Molekulare Membranbiologie Max Planck

[ccp4bb] Heme D restraints

Hey Everyone,I am having trouble generating a adequate cif restraint file for a heme d ligand.I have tried to do a couple of things now, but either the restraint file is simply wrong (Phenix suite) or the output is generating a restraint file without a complexed Fe atom (CCP4).Additionally I have u

Re: [ccp4bb] Tev cleavage

Hey Giulliana, does your protein buffer contain high imidazole concentrations ( > 150 mM)? If so you should try to exchange the buffer before cleavage, since the TEV protease tends to precipitate at higher imidazole concentrations. Schara > Am 02.05.2015 um 19:56 schrieb Giulliana Rangel : >

Re: [ccp4bb] Density modification issue

Hey Veera, you have missed to assign all labels which were required as input parameters. DM is trying to use your HLA labels for density modification. Either you assign them if present or you choose to omit these and just use FHI and FOM labels. This can be done in the header of the DM GUI. B