onversion. This may be another reason for discrepancies.
> Pavel
>
> On Fri, Apr 5, 2024 at 9:45 PM venkatareddy dadireddy <
> venkatda...@gmail.com> wrote:
>
>>
>> Thank you very much Garib.
>>
>> Venkat
>>
>> On Sat, Apr 6, 2024 at 1:12 AM
Thank you very much Garib.
Venkat
On Sat, Apr 6, 2024 at 1:12 AM Garib Murshudov
wrote:
> Unless you are confident that twin exists you should not use twin
> refinement (Occam’s razor)
>
>
>
> On 5 Apr 2024, at 17:24, venkatareddy dadireddy
> wrote:
>
> CAUTION:
was used then twin related intensities might have
> different resolution, in case when your crystal are pseudomerohedral
> twinned.
>
> Regards
> Garib
>
> On 4 Apr 2024, at 18:40, venkatareddy dadireddy
> wrote:
>
> CAUTION: This email originated from outside of th
you,
Venkat
On Tue, Apr 2, 2024 at 10:10 PM Kay Diederichs <
kay.diederi...@uni-konstanz.de> wrote:
> Hi Venkatareddy Dadireddy,
>
> do the unit cell parameters of your MTZ file and PDB file agree exactly ?
>
> Take for example a cell of (100,110,120,90,90,90) in the hea
the right resolution. What could be the possible reason for this
discrepancy? I have the structure deposited in the Protein Data Bank and it
is on hold. Thank you in advance for your help.
Thank you,
*Venkatareddy Dadireddy,B1-10,Prof. S. Ramakumar's Lab,Dept. of
Physics,IISc, Banglore.Cell
temperature.
I can just get a result of two single strand DNA/RNA. PAGE analysis.
No double helix was founded.
Does anyone have the same problem or know how to fix it.
Thank you for your answering.
Best regards,
Weifei
--
*Venkatareddy Dadireddy,B1-10,Prof. S. Ramakumar's Lab,Dept
Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: rrowl...@colgate.edu
On 1/22/2014 1:46 AM, venkatareddy dadireddy wrote:
Dear All,
My protein is cloned in pET-15b vector, contains
Hi,
I'm working on DNA binding protein, looking to co-crystallize protein- DNA
complex and have no previous experience. Your suggestion would be very
precious on the following queries.
1. My protein is 646 amino acid long and it exists as homodimer. It is also
having around 20 amino acid
and/ or Hoogstein base pairing.
2. If it binds self-complementary duplexes then try them also.
3. Peg conditions with slight acidic pH are more suitable.
4. Divalent cation salts (Ca, Mg, Mn) in crystallization.
Wish you good luck
Raj
On Friday, January 3, 2014, venkatareddy dadireddy wrote:
Hi,
I'm
