Regarding to amount of material loaded, I think it may also depend on
the buffer. When I did light scattering experiment with membrane
protein at angle of 90 degree, I got much better base line with buffer
containing DDM than one containing OG. This maybe due to high
concentration of OG required I
And as others have said shedding of dextran is a problem with GE columns
(this was confirmed to me by GE people), but after extensive system
equilibration, we do not see a problem significant enough to ever hurt
our light scattering measurements.
Engin
On 3/8/11 10:38 AM, Engin Özkan wrote:
they buy it from some small company, idea being the applicability to
SLS, optimized for minimized bleeding/sheding or material
from the column, which will show up only in the light scattering
detector.
I don't know. Every single spec of Wyatts columns looks exactly the same as
"Bio SEC-5" serie
On 3/8/11 5:03 AM, Sebastiano Pasqualato wrote:
On the other hand, GE Healthcare columns would require a huge amount of
material to be loaded.
What do you mean by a huge amount of material? You would not be using a
16/60 column (125 ml column volume) for an analytical experiment. How
about a
Hi,
they buy it from some small company, idea being the applicability to
SLS, optimized for minimized bleeding/sheding or material
from the column, which will show up only in the light scattering
detector.
but i have to say some of our proteins stick to their columns
(happened during the d
So from where does Wyatt outsource their columns?
JPK
On Tue, Mar 8, 2011 at 10:49 AM, Sally Pham Thanh Van
wrote:
> I should say clearer that I did a comparison between GE (Superdex 200
> 10/30) and the Wyatt column of the same separation range on AKTA
> connected with MALS. The Wyatt column g
I should say clearer that I did a comparison between GE (Superdex 200
10/30) and the Wyatt column of the same separation range on AKTA
connected with MALS. The Wyatt column gave better resolution for size
exclusion chromatography as well as signal-noise ratio for light
scattering. So I think it is
Hi Sebastiano,
we have used both silica based and superdex columns and we found the
second as a better option for general usage.
Most of our proteins are on basic buffer and about 50% of them stick on
the silica based column. On the other side a superdex 200 10/30 from GE
performed quite well,
://www.crystal.chem.uu.nl/group-gros/
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Sebastiano Pasqualato
Sent: martedì 8 marzo 2011 14:04
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] [OT] which column to use in SLS/MALS instruments
Dear all,
I was wondering if
Dear Sebastiano,
>From my experiences, Wyatt columns are the best.
Best wishes,
Sally.
On Tue, Mar 8, 2011 at 2:03 PM, Sebastiano Pasqualato
wrote:
> Dear all,
> I was wondering if somebody could help me out by suggesting the "best" column
> to be used in a Static Light Scattering (I guess it
Dear all,
I was wondering if somebody could help me out by suggesting the "best" column
to be used in a Static Light Scattering (I guess it would be the same for a
Multi Angle Light Scattering) instrument.
We were suggested using a silica-based column, with very high separation
properties, but i
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