@JISCMAIL.AC.UK] Im Auftrag von Enrico
Stura
Gesendet: Dienstag, 27. August 2013 11:58
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] AW: [ccp4bb] cryoprotection
Herman,
The trick you suggest is not as valid as you may think. The ice rings can
originate from the crystal itself.
If you crystallize in
Herman,
The trick you suggest is not as valid as you may think. The ice rings can
originate from the crystal itself.
If you crystallize in a high concentration PEG precipitant you will avoid
ice rings,
but if you transfer or soak your crystals in the same solution the high
molecular weight
A trick I like is just to freeze the reservoir solution or would-be
cryo-solution without a crystal present. If the frozen solution stays clear and
does not show ice rings on e.g. a home source, it is worth trying. Otherwise,
the solution needs optimization.
Herman
-Ursprüngliche Nachrich