A trick I like is just to freeze the reservoir solution or would-be cryo-solution without a crystal present. If the frozen solution stays clear and does not show ice rings on e.g. a home source, it is worth trying. Otherwise, the solution needs optimization. Herman
-----Ursprüngliche Nachricht----- Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Uday Kumar Gesendet: Freitag, 23. August 2013 19:52 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] cryoprotection Hello Can anyone suggest a cryoprotectant for the following crystallization condition 0.2-0.4M sodium formate ~20% PEG 3350 0-25 mM Nickel 0-100 mM Malonate Thank you with regards uday
