Sorry for the off topic. I'm looking for a way to monitor
protein-(potential) ligand interaction. The ligand is small molecule
(mw~250) and we're looking for its potential interaction with couple human
proteins. (We do not know this small molecule interacts with these human
protein or not.)
Is
: Mon, 13 Jan 2014 21:50:07 +0900
From: hojunle...@gmail.com
Subject: [ccp4bb] Assays for protein-ligand interaction?
To: CCP4BB@JISCMAIL.AC.UK
Sorry for the off topic. I'm looking for a way to monitor protein-(potential)
ligand interaction. The ligand is small molecule (mw~250) and we're looking
FRET， CD， Fluorescence， NMR chemical shift assay, isotope-labelled ligand
interaction assay, protein melting temperature assay, gel filtration retention
assay, gyration radius assay by Malls, native page gel analysis, etc.
Acoot
On Monday, 13 January 2014 8:51 PM, HJ Lee
fluorescence needs very low concentration of protein (nM to microM)
george
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Acoot
Brett
Sent: Monday, January 13, 2014 3:09 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Assays for protein-ligand interaction?
FRET， CD
