acharyya.iit...@gmail.com]
Sent: Friday, February 13, 2015 3:50 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Can nucleoprotein complex inhibit EtBr binding?
Dear Community,
Sorry for this off topic question. I am dealing with a non specific DNA binding
protein. In a non radio-labelled E
What is the charge on your protein? If it is a very positively charged protein
it may not enter the gel. I have found this before in EMSAs but normally at low
protein concentrations it still enters the gel only at the higher concs it does
not. I had to change protein to DNA ratio and also the pe
Sudipta,
the link for the cyanin dyes are at the following link
http://www.lifetechnologies.com/us/en/home/references/molecular-probes-the-handbook/nucleic-acid-detection-and-genomics-technology/nucleic-acid-stains.html
Sorry for the wrong link
P
On Fri, Feb 13, 2015 at 1:50 PM, Sudipta Bhattacha
Dear Community,
Sorry for this off topic question. I am dealing with a non specific DNA
binding protein. In a non radio-labelled EMSA DNA:protein titration
experiment when I EtBr stained the 5% native acrylamide gel, I could see
the DNA control (101 bps) but as the amount of my protein increases I
