Re: [ccp4bb] Co-purified ligand present in protein crystal

Dear Wenhe, we had a similar case and extraction using CHCl3 plus CH3OH (2:1 ratio, v/v) (65 °C, 30 min) before mass spectrometry worked very well: https://www.ncbi.nlm.nih.gov/pubmed/21743455 Hope that helps, Tomas On Mon, Aug 21, 2017 at 4:41 AM, WENHE ZHONG

Re: [ccp4bb] Co-purified ligand present in protein crystal

Wenhe Potentially, mass spectrometry in combination with electron capture dissociation and/or collision-induced dissociation might be helpful. See doi: 10.1007/s13361-013-0662-5. Best wishes, Klaus === Dr. Klaus Fütterer

Re: [ccp4bb] Co-purified ligand present in protein crystal

Dear Wenhe, Are there other ligands known for this protein ? Could these be chemically modified and still interact with your protein ? Then you could use ligand affinity chromatography to purify your protein eluting with the same or other ligands. For me this the only way to obtain

[ccp4bb] Co-purified ligand present in protein crystal

Dear CCP4BB members, We would like to identify a ligand that is present in crystal structure (according to strong positive densities at active site) but absent in crystallization condition. We already have some candidates in mind based on our knowledges on this protein but we need to validate