I presume you have done density modification after calculating the exptl
phases?
If you use REFMAC to refine your partial model with the exptl/density
modified phase restraints your FWT PHWT fourier coefficients already use
the combined phase.
I am not sure how to choose a damping factor. If y
Hi,
I have tried SHARP, but no positive results could be obtained.
Do you have some articles on how to combine the model phase and experimental
phases?
I could only seen the consecutive density in mir 8 to 6 A resolution map, so
I could only locate roughly the position of helix.
After that I use
Hello,
sorry to appear too much last days but since people continue to comment the
problem of Alphar, my guess is that it might be a nice illustration to what
was discussed this week-end.
Since the crystal is very long in the c-direction (380 A) and relatively
short in two others (50 A), I su
Dear alphar~
... I guess that a lot of the subsequent advice depends to the counter-
question "Which program did you use for phasing "?
My view on the subject is summarized in:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Experimental_phasing
[any contributions are welcome to i
Hi Alphar,
A while ago I had success with a poor MIR map by building in a few
fragments I could identify and then combining phases from this model
with the MIR phases using SIGMAA (CCP4 program) and then calculating
new maps. A couple of cycles of this resulted in phases in which I had
con
Thanks, Ezra!
I have a native dataset.
I'll try what you said, Hope it works in my case.
Thanks!
Alphar
2009/2/21 Ezra Peisach
> Personally, I would try to build into what you see and then do phase
> recombination. I had a SeMet crystal in which I could only see part of a
> sheet and helix
Thanks, Poul!
How could I change the scale of the data intensities by a factor 2-3? Do I
need to change it in scaleit or to change it in mlphare?
Is that always necessary to make the occupancy of the major site to become
~1? I'll try this.
I use phenix to detect the twin effect, the results turned
Hi alphar~
Check the sites you get from anomalous difference Fouriers and stick
to those as your correct HA sites. At the same time you may consider
not to refine the HA substructure on the anomalous differences but
merely using the isomorphous differences. Maybe you need to change the
sca
Hi everyone!
I have a question on my poor MIR map.
Four datasets (two AU and two HG) were used for phasing upto 3 A resolution
in my case. I could locate several heavy atom sites for each dataset with
occupancy finally refined to about 0.3 to 0.4, and with B value refined to
about 50 to 80. I did
