Re: [ccp4bb] Density Joining Cysteine and Lysine Side chains

2008-05-22 Thread David Briggs
...by lower wavelength, I mean longer wavelength... or lower energy. Take your pick. Dave 2008/5/22 David Briggs <[EMAIL PROTECTED]>: > Hi Andrew > You don't say what your protein is crystallised in, or has seen during > purification, but maybe S- MERCAPTOCYSTEINE might fit? > (search for CSS in

Re: [ccp4bb] Density Joining Cysteine and Lysine Side chains

2008-05-22 Thread David Briggs
Hi Andrew You don't say what your protein is crystallised in, or has seen during purification, but maybe S- MERCAPTOCYSTEINE might fit? (search for CSS in MSD CHEM) Do you have lower wavelength data that you might get a sulphur anomalous signal to check this? S-methyl Cysteine might fit as well -

[ccp4bb] Density Joining Cysteine and Lysine Side chains

2008-05-22 Thread Andrew Gulick
Greetings. I'm wondering if anyone has ever seen something like this. I have a 2.1 A data set (synchrotron data) that is nearing completion. I see density that appears to join a cysteine side chain to a lysine (30 residues away from each other in primary sequence). There is a picture of the densit