Thanks Jim and Bert for all your suggestions. I plan to increase time
and/or amount of DDM and see what happens before switching detergents. I'll
keep the suggestions about other detergents and 96-detergent screen in the
back of my mind.
Bert, I noted your suggestion of also using a larger amount
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Raji
Edayathumangalam [r...@brandeis.edu]
Sent: Wednesday, July 10, 2013 10:23 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Detergent solubilization step (membrane proteins)
Dear BBers,
Sorry for the
edu]
Sent: Wednesday, July 10, 2013 10:23 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Detergent solubilization step (membrane proteins)
Dear BBers,
Sorry for the non-ccp4 post.
I'd like to hear tips and suggestions from the membrane protein folks in the
community.
I am currently purifyi
Raji,
First, I would suggest increasing the solubilization time by attempting
solubilization overnight at 4 degrees. I would also suggest a few more
detergent choices. In addition to DDM, my other favories are OG and
Elugent (a mixture of several detergents) for extraction from *E.
coli* membran
Dear BBers,
Sorry for the non-ccp4 post.
I'd like to hear tips and suggestions from the membrane protein folks in
the community.
I am currently purifying two different membrane proteins expressed in E.
coli. While I am able to extract practically all of my first protein from
the cell membrane in
