Hi,
I am assuming the enzyme is not active, or by substrate you do not mean the
actual substrate, may be an analogue. The substrate might be converted into
product and the leave through the channel and you will not find anything
bound to it. But I think you have taken care of that.
On Fri, Apr
Dear Bulletin Board,
I am trying to soak substrate into crystals of an enzyme, but so far I
can't see the substrate in the structure. Does anyone knows a program
to ensure that the entrance to the central cavity is accessibly? I
mean based on the whole crystal. I already checked the crystal
:10.1107/S0907444906047020)
HTH
Carsten
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Paul Lindblom
Sent: Friday, April 09, 2010 5:15 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Does the substrate has access to the active site
It is also possible that mother liquor prevents binding (although often
in such cases you would see some precipitant component in the active
site.
I would generally bet on need for conformational change. And you expect
to see the product complex, right?
Ed.
On Fri, 2010-04-09 at 11:15 +0200,
