CCP4 bulletin board [ccp...@jiscmail.ac.uk] On Behalf Of Narayanan
Ramasubbu [ramas...@umdnj.edu]
Sent: 10 September 2009 01:36
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Help with improving these crystals
Dear All:
We have been struggling to improve the crystals shown in the attachment.
Beyond improvement via 'chemical' means, I would heartily recommend
mutagenesis for crystal packing improvement. I would be glad to help you
with the latter if you're interested.
Artem
> Dear All:
> We have been struggling to improve the crystals shown in the attachment.
> These crystals form fro
Have you tried microseeding or macroseeding? The original crystals
for my Ph D project only diffracted to ~8 angstroms. Microseeding
gave crystals that eventually diffracted to ~2.0.
Here are two refs that might be useful:
Bergfors, T. (2003). "Seeds to crystals." J Struct Biol 142(1): 66-76.