Dear Matt,
We were working with Hal2p a lithium inhibited Ins-mono-PPase like protein 10
years ago.
We had good biochemistry showing that lithium replaced mg at the active site.
We grow crystals in presence of Li and we worked with 1.6 A diffraction data.
Unfortunatelly we did not see any el
Hi Ed -
There was a peak in the difference maps, as I recall. I believe it
initially got built as a water, but that proved to be too many
electrons, giving a negative peak. I removed the water, but it was
clear that something needed to be there, at which point I started
casting about for al
Matt,
thank you, this is an excellent summary. One question remains - the
lithium peak should be, afaiu, much lower than the water/sodium. Was
there a peak in difference map or was placement based on identifying
something that looked like a coordination site?
Cheers,
Ed.
On Thu, 2012-01-12 at
On 1/12/12 9:42 AM, Ed Pozharski wrote:
On Thu, 2012-01-12 at 09:52 +, Patel, Joe wrote:
Do you have ultra-high resolution? Something I did not…. Are there
many examples in the pdb of proteins with Li+ refined?
http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=n
On Thu, 2012-01-12 at 09:52 +, Patel, Joe wrote:
> Do you have ultra-high resolution? Something I did not…. Are there
> many examples in the pdb of proteins with Li+ refined?
http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=n/a&template=het2pdb.html¶m1=_LI
39 in
.com <mailto:name.surn...@astrazeneca.com>
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From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Scott Pegan
Sent: 12 January 2012 06:10
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Lithium versus Sodium
Hey
Hey all,
Does anyone know of a good article that deals with differentiating between
a lithium ion and sodium ion for density in a X-ray structures?
Scott
--
Scott D. Pegan, Ph.D.
Assistant Professor
Chemistry & Biochemistry
University of Denver
Office: 303 871 2533
Fax: 303 871 2254
