I have the same problem as I am not using a robot.
For this reason I use the 48 well hanging drops from Hampton and seal them
one at a time. It is very time consuming but uses little reservoir
solution and your drops do not dry out..
Jackie Vitali
On Fri, Nov 18, 2011 at 7:24 PM, Ho Leung Ng w
it can be done but, if you don't want your drops to dry, better do it in a cold
room. It's painful though.
Hello Ivan,
You can dispense 0.1-0.2 uL drops with the PB-100 repeating
dispenser from Hamilton and a 10 uL glass syringe. But it's rather
clumsy and hard on the hands. I don't recommend using it with 96 well
plates. It's too slow to do the whole plate at one time without your
drops drying up
Hi,
With the electronic Biohit 8 channel pipette you can set up 3x96 drops in 5
minutes. Lowest volume is 0.2 ul. We have used this system for a couple of
years with RNase free filter tips for RNA-protein complexes. With the recent
arrival of a shared Mosquito we have used the Biohit less but it
On Fri, 2011-11-18 at 06:34 -0800, xaravich ivan wrote:
> Ok, now I do not have an easy access to crystallization robot, so I
> was hoping if someone here have ever used the 96 well plates for
> manually setting drops with much lower solution/sample volumes
> (0.1-0.2micro litres).
>
The best we
sorry totally misunderstood your question, however if you can ship your
protein i can always try to setup a tray for you.
Shya
On Fri, Nov 18, 2011 at 9:34 AM, xaravich ivan wrote:
> Hi,
> I apologize in advance as it is not a ccp4 related question, but over the
> years, CCP4bb is synonymous with
Hi,
I am routinely using the gryphon robot from Art Robbins.This instrument can
dispense 0.02microlitre at minimum. Your drops can dry out if you use such
low volumes you have to be really fast. You can set up 0.2 to 0.1 micro
litre drops using this for 96 well plates however the instrument needs t
Hi,
I apologize in advance as it is not a ccp4 related question, but over the
years, CCP4bb is synonymous with protein crystallographers virtual
university, at least for me.
Ok, now I do not have an easy access to crystallization robot, so I was
hoping if someone here have ever used the 96 well pl
