Hi Devbrat,Have you tried indexing using XDS multi-lattice processing? It is a very useful indexing process for multi-lattice and/or 2nd lattice interference with the major one. Best,Rezaul Karim, Ph.D.Structural Biology ScientistNurix Therapeutics Inc.Sent from my iPhoneOn Nov 15, 2023, at 6:22
Dear Prof John
Thank you for your valuable time and insight. I will do as you have
suggested.
*Warm Regards-*
*Devbrat Kumar*
On Wed, Nov 15, 2023 at 11:28 PM John Bacik <
b45abf420e1f-dmarc-requ...@jiscmail.ac.uk> wrote:
>
> Hi Devbrat, here are a couple of other things to try:
>
> -
Dear Prof Gerard
Thank you for your in-depth analysis of the possibilities I am facing. The
image slicing was at 0.1 degrees.
*Warm Regards-*
*Devbrat Kumar*
On Wed, Nov 15, 2023 at 5:21 PM Gerard Bricogne
wrote:
> Dear Devbrat,
>
> With the unit-cell geometry you have, namely a very
Dear Professor,
Thank you for your valuable suggestions.
I have attempted to investigate the NCS operator, but it seems to be a
monomer. Nevertheless, I will reconsider your suggestion regarding this
matter.
I employed the concept of using high-resolution data (2 Angstroms) without
cutoff for
Hello Professor Kay
Thank you for your in-depth and valuable suggestions.
1. I used to observe multiple spots at one Phi, and at 90 degrees, I would
find a streak of spots. The crystal used to be spade-shaped. The spots are
visible in a count of 8 (matching the total number of crystal lattices).
Dear Professor Phil
Thank you for highlighting and providing input. Yes, The space group is P21.
*Warm Regards-*
*Devbrat Kumar*
On Wed, Nov 15, 2023 at 5:39 PM Phil Evans wrote:
> Is the space group really P2? P21 is MUCH more common
> Phil
>
> > On 14 Nov 2023, at 15:55, Devbrat Kumar
Hi Devbrat, here are a couple of other things to try:
- When screening crystals use rastering to find regions of the sample where
multiple lattices may be less problematic. If multiple lattices are observed,
often regions on the crystal(s) close to the edge will not be as affected by
Is the space group really P2? P21 is MUCH more common
Phil
> On 14 Nov 2023, at 15:55, Devbrat Kumar wrote:
>
> sed data were integrated with the data reduction tool AIMLESS in the CCP4i2
> suite.
To unsubscribe from
Dear Devbrat,
With the unit-cell geometry you have, namely a very long axis and two
much shorter ones, you have to be exceedingly careful about how the data are
collected, and in particular about the crystal orientation and image width.
If the long axis can be brought close to being
You seem pretty near to having solved your structure!
Ignoring the data problems..
Extra steps I might have used.
1) Self rotation function. (in CCP4I2 the task is under data analysis ..)
Does it suggest a NCS operator?
If so is this a two fold? which might mean you have a dimer..
2) Now you
Hello Devbrat,
your project is difficult and there is no magic bullet to solve its problems.
Your approach is good because it always pays off to process
the data carefully.
In this respect, let me make a few comments.
1) you don't say why you call the diffraction patterns "multi-lattice". What
Hello everyone,
The issue with the crystal is its multi-lattice nature; even the truncated
protein, which has been crystallized, exhibits multi-lattice
characteristics (detectable only after XRD).
I have multiple native and selenium datasets with similar unit cell
parameters. (One axis is
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