Dear All,
This may be slightly off-the-track question but your feedback will be very much
appreciated. The situation is-
I obtain a very low amount of the protein of my interest (a hetro-dimer) from
the construct I am using (only 8% of the total amount of protein obtained is
the protein of my
with Circular dichroisme or DLS).
Hope to help you.
Nicolas
De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Anindito Sen
[andysen.to...@gmail.com]
Envoyé : jeudi 30 janvier 2014 14:17
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] Protein
Do you really need to remove the NaCl? Some ionic strength is often
necessary to stabilize proteins. Our routine purification buffers all
contain at least 100 mM NaCl. This will not usually interfere with
crystallization screening.
To minimize the probability of aggregation, you need to (1)
Dear All,
1st of all , Thanks for the very quick feedback. I will answer your questions
to make the picture more clear-
Nicolas-
Yes the dimer is co-expressed. Almost all the protein is in the sup and not in
the pellet. Multiple-step dialysis has failed. I have been trying various
controls
