Hi all,
I have a naive question about antibodies. Many people used Fab fragments in
crystallization. I am wondering if it is possible to use the whole IgG
molecule with Fc fragment as well. Or it would be too flexible and bad for
crystallization?
Thanks,
Cheng
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Cheng Z
The fact that the PDB holds hundreds of FABs and a handful whole ABs
suggests to me that the latter are hard to get crystals for. So, all the
more reason for us bioinformaticians that you, experimentalists try it :-)
Gert
On 22-6-2017 20:39, Cheng Zhang wrote:
Hi all,
I have a naive questi
Yes, the hinge region between Fab and Fc is highly flexible. If you look at
IgG by EM you will see many different conformations. Best to use Fab or
scFv.
Jarrod Mousa
On Thu, Jun 22, 2017 at 1:39 PM, Cheng Zhang wrote:
> Hi all,
>
> I have a naive question about antibodies. Many people used Fab
