Good luck
>
> ** **
>
> Thierry
>
> ** **
>
> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *Uma
> Ratu
> *Sent:* Wednesday, February 13, 2013 5:38 PM
>
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* [ccp4bb] S-nitrosylation protein
&g
, depending on how fast the degradation occurs.
Good luck
Thierry
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Uma Ratu
Sent: Wednesday, February 13, 2013 5:38 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] S-nitrosylation protein
Dear All:
I plan to use X-ray
Dear Uma,
A very complehensive survey of the state-of-the-art in this topic can be found
in the following literature resources:
(1)
Garman EF.
Radiation damage in macromolecular crystallography: what is it and why should
we care?
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):339-51.
Maybe you can try different energies hoping that damage is wavelength
dependent. It must be dose dependent though, so you may consider merging short
sweeps from multiple crystals.
Original message
From: Uma Ratu
Date:
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] S
Dear All:
I plan to use X-ray crystallography method to study the S-nitrosylated
protein structure.
The native protein crystals diffracted to 2A with synchrontron. I now have
the crystals of S-ntrosylated protein.
Since S-NO moiety appears to be unstable to synchrotron radiation, could
you advic
