Hi Jacob,
check out Figure 1 in
Natively inhibited Trypanosoma brucei cathepsin B structure determined by using
an X-ray laser.
Redecke L, et al.
Science. 2013 Jan 11;339(6116):227-30.
and
In vivo protein crystallization opens new routes in structural biology.
Koopmann R. Nature Methods. 2012
Of course, common in baculo:
http://dx.doi.org/10.1038/emboj.2009.352
The EMBO Journal (2010) 29,505--514
*How baculovirus polyhedra fit square pegs into round holes to robustly
package viruses*
Xiaoyun Ji, Geoff Sutton, Gwyndaf Evans, Danny Axford, Robin Owen and
David I Stuart
On 15/
n Genetics
Division of Structural Biology
Roosevelt Drive, Oxford OX3 7BN
United Kingdom
Phone: +44-1865-287564
Fax: +44-1865-287547
Original message
>Date: Fri, 15 Feb 2013 14:44:32 -0500
>From: CCP4 bulletin board (on behalf of Jacob Keller
>)
>Subject: [ccp4bb] Sighting of Pr
://hmg.oxfordjournals.org/content/9/12/1779.long
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Frank von
Delft
Sent: Friday, February 15, 2013 3:01 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Sighting of Protein Crystals in Vivo?!
Of course
] Sighting of Protein Crystals in Vivo?!
Of course, common in baculo:
http://dx.doi.org/10.1038/emboj.2009.352
The EMBO Journal (2010) 29,505–514
How baculovirus polyhedra fit square pegs into round holes to robustly package
viruses
Xiaoyun Ji, Geoff Sutton, Gwyndaf Evans, Danny Axford,
that the inclusion bodies formed during over expression of
transgenic proteins in E. coli are crystalline. I expect that we will be
enlightened on this issue by somebody on the BB soon.
Zhijie
From: Jacob Keller
Sent: Friday, February 15, 2013 2:44 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [cc
i* are crystalline. I
> expect that we will be enlightened on this issue by somebody on the BB soon.
>
> Zhijie
>
>
>
> *From:* Jacob Keller
> *Sent:* Friday, February 15, 2013 2:44 PM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* [ccp4bb] Sighting of Protein Crystals in V
7564
Fax: +44-1865-287547
Original message
>Date: Sat, 16 Feb 2013 15:38:12 -0600
>From: CCP4 bulletin board (on behalf of Artem
>Evdokimov )
>Subject: Re: [ccp4bb] Sighting of Protein Crystals in Vivo?!
>To: CCP4BB@JISCMAIL.AC.UK
>
> In addition to the a
man Genetics
> Division of Structural Biology
> Roosevelt Drive, Oxford OX3 7BN
> United Kingdom
> Phone: +44-1865-287564
> Fax: +44-1865-287547
>
>
> Original message ----
> >Date: Sat, 16 Feb 2013 15:38:12 -0600
> >From: CCP4 bulletin board (on beha
>Date: Sat, 16 Feb 2013 16:14:58 -0600
>From: CCP4 bulletin board (on behalf of Artem
>Evdokimov )
>Subject: Re: [ccp4bb] Sighting of Protein Crystals in Vivo?!
>To: CCP4BB@JISCMAIL.AC.UK
>
> Note: the crystals in my paper are not regular GFP,
> they're special
Jacob, I recall Dorothy Hodgkin or Guy Dodson showing a slide (mid to late
1970s) with insulin crystals having grown in the Islets of Langerhans. As you
say, quite remarkable. John
Prof John R Helliwell DSc
On 15 Feb 2013, at 19:44, Jacob Keller wrote:
> Dear Crystallographers,
>
> I
On 02/16/13 00:46, Zhijie Li wrote:
Hi Jacob,
Interesting topic.
This reminds me the posters I saw on ACA 2010, on the femto-second
infrared laser based instrument . That instrument utilizes the
nonlinear optical properties of crystals of chiral molecules to
detect very small crystalline mate
Dear Jacob,
As mentioned before by Frank, many insect viruses use in vivo crystals as
their main infectious form:
*The molecular organization of cypovirus polyhedra.* Nature 446, 97–101
(2007). Coulibaly, F. et al.
*The atomic structure of baculovirus polyhedra reveals the independent
emergence of
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