[ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?)

2015-01-12 Thread Keller, Jacob
>the top-hat profile is one of the reasons why inhouse machines produce better >quality data than synchrotrons. However, the often much increased resolution >you achieve at the synchrotron is generally worth more than the quality of the >data at restricted resolution. > >Cheers, >Tim Several su

Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?)

2015-01-12 Thread Tim Gruene
Hi Jacob, at the beginning of my experience of S-SAD about 10 years ago, it was not too difficult to do S-SAD phasing with inhouse data provided the resolution was better than 2.0A, while it did not always work with synchrotron data. Purely personal experience. However, the inhouse machines I am

Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?)

2015-01-12 Thread Keller, Jacob
>at the beginning of my experience of S-SAD about 10 years ago, it was not too >difficult to do S-SAD phasing with inhouse data provided the resolution was >better than 2.0A, while it did not always work with synchrotron data. Purely >personal experience. I assume that the synchrotron data were

Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?)

2015-01-13 Thread Andrew Leslie
Hi Tim, Jacob, I must admit that I was very surprised by the suggestion of a top-hat profile for a in-house rotating anode. We have a Rigaku Fr-E generator, and Rigaku provided a plot of the beam profile for that (with VariMax-HR Optic) and it is very far from being top

Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?)

2015-01-14 Thread Tim Gruene
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Hi Jacob, both George Sheldrick and Andrew Leslie explained to me that the machine I had in mind - a sealed tube generator with a graphite monochromator - is not really state of the art and merely a technology from 20 years ago. Hence my comment about

Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?)

2015-01-15 Thread James Holton
Jacob, Background subtraction is unfortunately not as forgiving as you think it is. You can subtract the background, but you can't subtract the noise. This is because noise (by definition) is never the same twice. Yes, the "average" or "true" background under a spot may be flat, but any give

Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?)

2015-01-15 Thread Dom Bellini
: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?) Jacob, Background subtraction is unfortunately not as forgiving as you think it is. You can subtract the background, but you can't subtract the noise. This is because noise (by definition) is never the

Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?)

2015-01-15 Thread Keller, Jacob
-Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Dom Bellini Sent: Thursday, January 15, 2015 12:25 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?) Dear Jacob, To add

Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?)

2015-01-15 Thread Keller, Jacob
ce at high intensity, for a given total dose! Jacob -Original Message- From: James Holton [mailto:jmhol...@lbl.gov] Sent: Thursday, January 15, 2015 12:00 PM To: Keller, Jacob; CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does ra

Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?)

2015-01-17 Thread James Holton
igh intensity, for a given total dose! Jacob -Original Message- From: James Holton [mailto:jmhol...@lbl.gov] Sent: Thursday, January 15, 2015 12:00 PM To: Keller, Jacob;CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?)