Hi Leonid,
Thank you for your valuable suggestion. It is exactly the case. When I
tried P21, it works well. The solution is now very clear.
Best,
Zhiyi
On 3/31/12, Leonid Sazanov saza...@mrc-mbu.cam.ac.uk wrote:
Hi, we had the same case in apparent C2221, with many similarly shifted
Phaser
When this happens there is usually a serious problem with the data.
Have you checked the truncate output for a non-cryst translation vector? It
would look as though you have something which is generating a pseudo
translation along x of ~ 0.2
Look at the hklview pictures of your data and see
Hi,
Do you mean that the second molecule is always overlapped with the first, by
saying that it shifts several Angstrom along the x axis? If there were a
larger translation, then what you're seeing would be consistent with
translational NCS (tNCS), but the translation should be large enough
Hi, we had the same case in apparent C2221, with many similarly shifted Phaser
solutions with high scores. The reason was that crystals were actually nearly
perfectly twinned in P21, so indexing and processing indicated C2221. Once data
was re-processed in P21, Phaser could easily find two
Dear all,
I got a weird solution from Phaser. The background is that, space
group C2221, resolution ~4A, in complex with a peptide, and having a
apo form structure as the search model. Phaser gave two rotation
function peaks with Z 7. But when searching translation function
peaks, Phaser gave
