king for a reference for cacodylate decomposition in protein
crystals upon X-ray exposure
Hi!
I heard a couple of times that use of cacodylate buffers in crystallization
is bad, and not only because of the compound toxicity.
As I understood, presence of the cacodylate in a protein crystal wil
Hi Tanya - did you read the next message in the thread you posted? It
answers the question, even if somewhat succinctly:
http://www.proteincrystallography.org/ccp4bb/message23692.html
The April 2011 issue of Journal of Synchrotron Radiation has the
proceedings from the 2010 Radiation Damage wo
I do not have the reference you are seeking, but I have seen
cacodylate-containing xtals diffract to better than 1.2 and hold up very
well. Also, arsenic has an anomalous signal which may be exploited for
phasing, peak ~ 1.04 A.
On 07/29/12 18:53, Tatyana Sysoeva wrote:
Hi!
I heard a couple
Dear Tatyana,
We once had a project where the crystallization
condition contained cacodylate. The crystals diffracted
to 1.9A and survived reasonably well under the beam
(maybe there was indeed some colour change upon
exposure, I do not remember exactly).
We were working with drug soaks. The ori
Am 29 Jul 2012 um 18:53 hat Tatyana Sysoeva geschrieben:
>
> Hi!
>
> I heard a couple of times that use of cacodylate buffers in crystallization
> is bad, and not only
> because of the compound toxicity.
>
> As I understood, presence of the cacodylate in a protein crystal will cause a
> part
Hi!
I heard a couple of times that use of cacodylate buffers in crystallization
is bad, and not only because of the compound toxicity.
As I understood, presence of the cacodylate in a protein crystal will cause
a particular crystal degradation pattern upon X-ray exposure - "darkening
of the cryst
