Hi all,
Sorry for the very off topic problem. I'm looking for a loading control for my
westerns (doing in vitro assays). Due to separation/antibody specificity
issues, I need super good separation between 70 and 100 kDa, oftentimes running
those markers all the way to the bottom of the gel. If
How about plain old BSA ?
Jürgen
On Apr 26, 2012, at 1:29 PM, Peter Hsu wrote:
Hi all,
Sorry for the very off topic problem. I'm looking for a loading control for my
westerns (doing in vitro assays). Due to separation/antibody specificity
issues, I need super good separation between 70 and 10
Just in case you are blocking with BSA, how about, e. coli
beta-galactosidase (114 KDa) ?. It's commercially available but you
probably have cells already making it in the lab
On 26 April 2012 19:29, Peter Hsu wrote:
> Hi all,
>
> Sorry for the very off topic problem. I'm looking for a loading