Hi Ursula,
you could try Perfluoropolyether PFO-X175/08 (Hampton Research HR2-814). You
just plunge your crystals in the liquid or wipe the crystal in the loop through
the liquid so that all excess water is removed.
Good luck !
Ulrike
protection for low salt crystallization at 4
degrees
Date: Mon, 2 Mar 2015 20:32:47 +0100
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Dear Ursula,
unless it is too long a list, maybe you could list the
cryo-protectants you have used so far? Did you try sugars (e.g.
glucose), or Na Malonate
bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of
*Ursula
Schulze-Gahmen
*Sent:* Monday, March 02, 2015 1:49 PM
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] cryo protection for low salt crystallization at 4
degrees
I know there was jut recently a discussion about cryoconditions
I know there was jut recently a discussion about cryoconditions for
crystals, but I am still hoping for some new ideas for my crystals that
grow from HEPES buffer pH 7.3, 0.2 M NaCl by slowly lowering the
temperature from 20 to 4 degrees.
These crystals are easy to grow but extremely sensitive to
-BEGIN PGP SIGNED MESSAGE-
Hash: SHA1
Dear Ursula,
unless it is too long a list, maybe you could list the
cryo-protectants you have used so far? Did you try sugars (e.g.
glucose), or Na Malonate, or Butanediol (either 2,5 or 1,6, I don't
remember exactly)?
You could also increase the
What about 4deg data collection?
What about glutaraldehyde crosslinking?
JPK
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Ursula
Schulze-Gahmen
Sent: Monday, March 02, 2015 1:49 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] cryo protection for low salt crystallization
