Re: [ccp4bb] effects of salt on twinned crystals

2012-02-22 Thread Ms Chiung-Wen Chang
23, 2012 4:06 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] effects of salt on twinned crystals I read your description of the crystals and Figure 4 of the following paper came to mind. Post-crystallization treatment in lower PEG eventually allowed them to tease the bundles apart. 1

Re: [ccp4bb] effects of salt on twinned crystals

2012-02-22 Thread Francis E Reyes
I read your description of the crystals and Figure 4 of the following paper came to mind. Post-crystallization treatment in lower PEG eventually allowed them to tease the bundles apart. 1. MacRae, I. J. & Doudna, J. A. An unusual case of pseudo-merohedral twinning in orthorhombic crysta

Re: [ccp4bb] effects of salt on twinned crystals

2012-02-22 Thread Peter Hsu
Forgot to mention, that this 2.5-3A diffracting crystal was the same one that I have been unable to index and suspect are twinned due to the presence of these other fused crystals in the same drop. Thanks for any input.

[ccp4bb] effects of salt on twinned crystals

2012-02-21 Thread Peter Hsu
Hi all, Thanks to all those who responded to my query about indexing my dataset. I'm beginning to suspect that the crystals are twinned. the crystals initially started as fused plates which were then optimized to single crystals that diffracted poorly (3.5-4). I then set up an additive screen,