Hi all,
I was intrigued by the recent question of whether glycerol had any adverse
effects on the final purity of protein isolated by chromatography. Glycerol
certainly helps to solubilize some proteins. Does anyone know of any negative
effects of glycerol in protein purification, on protein cr
Ray Brown
Sent: Thu 10/03/2011 17:04
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] glycerol
Hi all,
I was intrigued by the recent question of whether glycerol had any adverse
effects on the final purity of protein isolated by chromatography. Glycerol
certainly helps to solubilize some proteins
the first additives to try.
_
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Ray
Brown
Sent: Thursday, March 10, 2011 4:05 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] glycerol
Hi all,
I was intrigued by the recent question of whether glycerol had any adverse
Hi Ray;
In case of my protein 5%-10% of glycerol help to increase the solubility
both of the soluble domain as well as the trans-membrane domain,improve
crystal quality dramatically and even prevent radiation damage to some
extent.
Best of luck
Bashir
On Thu, March 10, 2011 23:04, Ray Brown w
*From:* CCP4 bulletin board on behalf of Ray Brown
*Sent:* Thu 10/03/2011 17:04
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] glycerol
Hi all,
I was intrigued by the recent question of whether glycerol had any
adverse effects on the final purity of protein isolated by
chromatography
o:* CCP4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] glycerol
Hi all,
I was intrigued by the recent question of whether glycerol had any
adverse effects on the final purity of protein isolated by
chromatography. Glycerol certainly helps to solubilize some
proteins.
Does anyone know of any negative effects of g
Dear Ray,
The solubility and stability effect of glycerol is protein dependent and
highly concentration dependent. When used at the wrong concetration or
molar ratio to protein, preferential interaction could result in adverse
effects. Likewise at concentrations higher than 15% in drops for crysta
Hi.
I am trying to optimize a commercial crystallization condition that
contains glycerol. So far, I have not been able to even reproduce the
results from the initial screen, and I thought that this may be due to
poor quality glycerol (among other possibilities). I believe that the
glycerol that
I am working with a metalloprotein that binds cobalt and iron. I
was surprised that the solved structures showed the crystals
cryoprotected with glycerol are metal free while crystals
cryoprotected with ethylene glycol had the metals present. Both
cryoprotectant solutions contained metal in th
delete the
communication without retaining any copies. Thank you.
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Matthew Alan Bratkowski
Sent: Wednesday, April 01, 2009 9:04 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Glycerol for crystal
Ho,
interesting case.
Glycerol actually forms weak complexes with metal ions:
Journal of Inorganic Biochemistry,60 299-302 (1995) and some references
therein.
But to be true, I am surprised that glycerol at concentrations of 10-15%
(?) as used in cryo conditions can compete with a metal ion bin
e New Year's greetings to the Chickens no good
intentions are in his mind.
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Ho-Leung Ng
Sent: Wednesday, May 13, 2009 2:20 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Glycerol as metal chelator?
I wouldn't use a pH of 9 when dealing with metal cations in solution.
By and large, metal hydroxides precipitate at alkaline pH.
HTH
Pr. Nadir T. Mrabet
Cellular & Molecular Biochemistry
INSERM U-954
Nancy University, School of Medicine
9, Avenue de la Foret de Haye, BP 184
54505 V
Dear colleagues,
Has anyone seen glycerol on a three-fold axis?
THis is possible as the glycerol can be disordered but I want to know if
there is an actual case.
Any information would be greatly appreciated.
Jackie Vitali
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