Re: [ccp4bb] how to improve the diffraction quality of the crystals and avoid icerings

Hi Yuvraj, Here are some thoughts in addition to what's been suggested already: 1. A good recent reference is here: http://journals.iucr.org/f/issues/2015/06/00/en5564/index.html 2. The image you sent suggests cubic ice, indicating a higher glycerol concentration (maybe 35 or 40%) is needed.

Re: [ccp4bb] how to improve the diffraction quality of the crystals and avoid icerings

Hi Yuvaraj, the importance of proper cryo mounting is under-appreciated in the community. Intensities of reflections on or close to ice rings cannot be recovered by the data processing software with good accuracy, which is detrimental for structure solution and refinement. Thus it is vital to

Re: [ccp4bb] Fwd: [ccp4bb] how to improve the diffraction quality of the crystals and avoid icerings

Hi, Yuvraj, I can suggest two methods: 1) collect a dataset at room temperature 2) crystallize your protein in cryoconditions. That is add 25 % of glycerol to your crystallization conditions and perform small optimization in terms of precipitant concentration. 2017-12-29 7:55 GMT+03:00 Prem

[ccp4bb] Fwd: [ccp4bb] how to improve the diffraction quality of the crystals and avoid icerings

-- Forwarded message -- From: Prem Prakash Date: Fri, Dec 29, 2017 at 10:22 AM Subject: Re: [ccp4bb] how to improve the diffraction quality of the crystals and avoid icerings To: zaigham mahmood khan Hi, Yuvraj Initially I also

Re: [ccp4bb] how to improve the diffraction quality of the crystals and avoid icerings

Yuvraj This is a tough call. may be you need to look at this database: http://web.archive.org/web/20111011202903/http://idb.exst.jaxa.jp/db_data/protein/search-e.php Importantly, when you click on "ex" for the tab, "cryoprotectant", a window will pop up and you can read different kinds of