Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Tobias Beck
Dear all, dear John, Thanks for the replies and references! I like the ice cream tub setup. Best wishes, Tobias. On Thu, Oct 24, 2013 at 10:05 PM, Jrh jrhelliw...@gmail.com wrote: Dear Tobias, There is also this one :- http://dx.doi.org/10.1107/S0021889801007245 In this study we had to use

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Simon . Phillips
)    +44 (0)7884 436011 (mobile) www:   www.rc-harwell.ac.uk -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Derek Logan Sent: 24 October 2013 19:08 To: ccp4bb Subject: Re: [ccp4bb] largest protein crystal ever grown? Hi, Last spring I visited

[ccp4bb] השב: Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Boaz Shaanan
. Cheers, Boaz הודעה מקורית מאת: simon.phill...@rc-harwell.ac.uk תאריך: אל: CCP4BB@JISCMAIL.AC.UK נושא: Re: [ccp4bb] largest protein crystal ever grown? Hi Derek, That brings back memories. I am pretty certain that is the myoglobin crystal

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Felix Frolow
: [ccp4bb] largest protein crystal ever grown? Hi Derek, That brings back memories. I am pretty certain that is the myoglobin crystal that was already on Benno's shelf at Brookhaven when I went there in 1980 to collect my oxymyoglobin neutron data. It would the metmyoglobin crystal Benno

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Derek Logan
@JISCMAIL.AC.UK נושא: Re: [ccp4bb] largest protein crystal ever grown? Hi Derek, That brings back memories. I am pretty certain that is the myoglobin crystal that was already on Benno's shelf at Brookhaven when I went there in 1980 to collect my oxymyoglobin neutron data. It would the metmyoglobin

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Felix Frolow
: [ccp4bb] largest protein crystal ever grown? Hi Derek, That brings back memories. I am pretty certain that is the myoglobin crystal that was already on Benno's shelf at Brookhaven when I went there in 1980 to collect my oxymyoglobin neutron data. It would the metmyoglobin crystal

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread R. M. Garavito
limits. Cheers, Boaz הודעה מקורית מאת: simon.phill...@rc-harwell.ac.uk תאריך: אל: CCP4BB@JISCMAIL.AC.UK נושא: Re: [ccp4bb] largest protein crystal ever grown? Hi Derek, That brings back memories. I am pretty certain that is the myoglobin crystal

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Alexander Aleshin
: simon.phill...@rc-harwell.ac.ukmailto:simon.phill...@rc-harwell.ac.uk תאריך: אל: CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK נושא: Re: [ccp4bb] largest protein crystal ever grown? Hi Derek, That brings back memories. I am pretty certain that is the myoglobin crystal that was already on Benno's

[ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Tobias Beck
Dear all, I was just wondering if anyone has some information or references about the dimensions of the largest protein crystal ever grown? I am aware that for neutron protein crystallography one usually needs crystals with mm dimensions. I have found some information on crystallization under

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Frank von Delft
Very recently (last few months), 3x1x1mm monsters for neutrons. I think J. Crystal Growth. On 24/10/2013 16:33, Tobias Beck wrote: Dear all, I was just wondering if anyone has some information or references about the dimensions of the largest protein crystal ever grown? I am aware that for

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread William G. Scott
I remember seeing an approx (5mm)^3 haemoglobin crystal in the MRC LMB crystal growing room, and the nucleosome crystals there were almost as big in their longest dimension. Bill On Oct 24, 2013, at 8:33 AM, Tobias Beck tobiasb...@gmail.com wrote: Dear all, I was just wondering if anyone

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Simon . Phillips
@JISCMAIL.AC.UK] On Behalf Of Tobias Beck Sent: 24 October 2013 16:34 To: ccp4bb Subject: [ccp4bb] largest protein crystal ever grown? Dear all, I was just wondering if anyone has some information or references about the dimensions of the largest protein crystal ever grown? I am aware

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Jrh
Dear Tobias, Take a look at http://dx.doi.org/10.1107/S0108767389012912 The ribonuclease crystal I used to measure the speed of sound, using laser generated ultrasound, was of volume 129 mm3 ie 7.7x6.2x2.7 mm . David Moss of Birkbeck College provided it. Best wishes, John Prof John R Helliwell

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread David Briggs
Following on from John's comment, when I did my PhD at Birkbeck in the early 2000s, one of David Moss's other PhD students (John Bond) grew some gigantic (1cm edges) crystals of things like HEWL Myoglobin, which he then (somewhat perversely) crushed to load into capillaries for powder diffraction

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Victor Lamzin
Also following on from John's comment - back to the times of my PhD I was repeatedly growing crystals of bacterial formate dehydrogenase (80 kDa) of a size about 7x1.5x1 mm. I thought that was quite normal and did not even think of making a photo of 'just a protein crystal'. Victor

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Derek Logan
Hi, Last spring I visited the Protein Crystallography Station at Los Alamos. On a shelf, in a capillary in a serious exhibition-quality glass dome, was a crystal of myoglobin some 50 mm**3, if I remember correctly. I was told it had been made by Benno Schoenborn some decades earlier and had

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Jrh
Dear Tobias, There is also this one :- http://dx.doi.org/10.1107/S0021889801007245 In this study we had to use a smaller crystal than the largest ones available of 125mm3. They were a lovely rhombic dodecahedral crystal habit. Nb we only published details of the size of the one used. These