I calculated threefold averaged omit maps in coot. These maps look nice and
clean, but I am having trouble making sense of the displayed sigma levels
or e/A3 values. When I display the unaveraged and averaged maps at a
similar density level for the protein the unaveraged map is at 0.024 e/A3
and
Ursula,
please don't forget that you are looking at Fourier image of electron
density distribution, which may be as different from the true electron
density distribution as different from zero the F000 reflection and as
different your Fobs data set from being 100% complete for all possible
On 06/06/12 21:47, Ursula Schulze-Gahmen wrote:
I calculated threefold averaged omit maps in coot. These maps look
nice and clean, but I am having trouble making sense of the displayed
sigma levels or e/A3 values. When I display the unaveraged and
averaged maps at a similar density level for
I'm afraid I seriously mistrust the sigma and e/A^3 numbers reported
by Coot for ncs averaged maps. I work with a crystal with near perfect
6-fold ncs and the e/A^3 numbers make no sense. For a 2Fo-Fc style map
the e/A^3 values should be nearly the same after as before. They are not.
