Dear ALL,
I got two kinds of crystals of different proteins ,but there are many
similarities.
The shape of the crystals are similar, the cell parameters are also similar :
protein A , 136.12 94.398 89.47690 125.47990 , Space group C 1 2 1
and
protein B , 136.14 94.369 89.115
With those statistics, it seems most probable that these two crystals
are the same protein. Do your two target proteins share an expression
system and/or purification protocol that could lead to the same
contaminant in both? If you have the resolution you could try Arcimboldo
to get an initial
The cell dimensions are very suspicious, but try merging the two sets of
data to see if they really are the same - you can do that best from the
integrated data sets, or just compare the Riso between the two sets of
amplitudes.
I think there is a web server at CCP4 SIMBAD which searches to see if
Answer from Ronan Keegan:
he cell dimensions are very suspicious, but try merging the two sets of
data to see if they really are the same - you can do that best from the
integrated data sets, or just compare the Riso between the two sets of
amplitudes.
I think there is a web server at CCP4 SIMBA
The reduced (aka "Niggli") cell for your case is 82.8246 88.2997 82.8246
98.8892 110.5181 117.0456
You can get this using "tracer" or "othercell" in the CCP4 suite.
I keep a local table of reduced cells from the PDB that I update
periodically, and the closest one to yours in that list is:
4y
By the way. Does anyone out there have a unit cell search engine still
running? The two mentioned on this thread so far:
ContaMiner: https://strube.cbrc.kaust.edu.sa/contaminer/
and SIMBAD: http://ccp4serv7.rc-harwell.ac.uk:8080/testserv/
seem to both be down. And the other one I know
Try
http://iterate.sourceforge.net/sauc-1.0.0/
which finds 4Y42 as closest for both cells, but lots of others nearby.
On Sun, Jun 18, 2017 at 10:39 AM, James Holton
wrote:
>
> By the way. Does anyone out there have a unit cell search engine still
> running? The two mentioned on this thread s
