Hi Martyn,
When it comes to micro-dialysis, you could check the following paper:
http://www.jbc.org/content/260/25/13580.full.pdf+html
(freely available, check in particular the appendix for the
micro-dialysis setup that was used, that was for heat-labile enterotoxin)
Fred.
PS I know this is
Martyn Symmons
Cambridge
- Original Message
From: David Briggs
To: CCP4BB@JISCMAIL.AC.UK
Sent: Monday, 21 December, 2009 17:26:15
Subject: Re: [ccp4bb] where I have been going wrong in crystallization?
Hi Martyn,
this recipe tends to work for me...
Lysozyme: 50 mg/ml in 0.1 M Sodi
Maybe is a mistake by a non-English speaker. Instead of "respectfully" should
say
"respectively"
t to the person who wrote down the recipe, but as we all
> know, what it obvious to one person, is not obvious to the next - especially
> in such difficult things like respect. Good recipes are indispensable and
> should be explicit about such important ingredients.
>
> Mark
>
>
obvious to the next - especially in
> such difficult things like respect. Good recipes are indispensable and should
> be explicit about such important ingredients.
>
> Mark
>
>
> -Original Message-
> From: Mark J. van Raaij
> To: CCP4BB@JISCMAIL.AC.U
ct. Good recipes are indispensable and should be
explicit about such important ingredients.
Mark
-Original Message-
From: Mark J. van Raaij
To: CCP4BB@JISCMAIL.AC.UK
Sent: Mon, Dec 21, 2009 12:18 pm
Subject: Re: [ccp4bb] where I have been going wrong in crystallization?
- I think the orig
- I think the original poster was only calling attention to the fact
that some proteins want to be treated respectfully in order to
crystallise (and the fact that Rigaku Japan realises this). I find
that indeed the case.
Other proteins, however, prefer the attitude "I don't know why I am
se
I was recently testing out the 30% PEGMME 5k, 0.1M NaOAc pH 4.5, 1M
NaCl method mentioned in a Hampton Research catalog and attributed to
Enrico Stura. I see that he has also just commented on this thread.
I found that at 80mg/ml, batch with 1.5ul:1.5ul protein to precipitant
ratio, lysoz
Dear Martin,
The original procedure with polyethylene glycol comes from:
Stura, E.A. (1998) Strategy 3: Reverse Screening. In "Crystallization of
Proteins: Techniques, Strategies and Tips. A laboratory manual" (Bergfors,
T., ed) International University Line. pp. 113-124.
If you follow th
Hi Martyn,
this recipe tends to work for me...
Lysozyme: 50 mg/ml in 0.1 M Sodium Acetate pH 4.8
Reagent: 8% w/v Sodium Chloride, 0.1 M Sodium Acetate pH 4.8
Additional Reagents: Index Reagent 8, 22, 28, 31, 34, 40, 58, 59, 69, 86, 88
Mix equal amounts of lysozyme with reagent, incubate at 4 or
Dear All
checking out the Lysozyme crystallization methods on the web I liked the
Rigaku Instructions that I found:
(http://www.rigaku.com/protein/crystallization.html)
"...create a drop of 3ul lysozyme solution, and 3 ul of well solution,
respectfully, for a total drop size of 6ul..."
So
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