[Histonet] Cryosectioning dorsal mouse skin

Greetings all, I'm wondering whether there are any recommendations for how to effectively cryosection dorsal mouse skin for immunohistochemistry. I'm having difficulty with much of the mouse skin slipping off the slide during the fixation and washing steps, which seems to result in loss of the

Re: [Histonet] BioCare

This happens to be my favorite of all the newer instruments. If it does everything proposed-wow, what a time saver it would be. To be able to load it without the other program being finished. DAKO tried an instrument like this a couple of years ago but it bombed. I would set yourself up for

[Histonet] BioCare

Did anyone see the new biocare IHC stainer at NSH? could you give your impressions?  We are looking at the Bond, Leica Bond, but we have just heard about the biocare system.   Thanks in advance, ___ Histonet mailing list Histonet@lists.utsouthwestern

Re: [Histonet] Cassette Holders

Jennifer-   Be of good cheer!   I saw them at the NSH convention.   Now if I could only remember where...   Joe - Original Message From: "[EMAIL PROTECTED]" <[EMAIL PROTECTED]> To: Histonet@lists.utsouthwestern.edu Sent: Thursday, September 18, 2008 3:47:34 PM Subject: [Histonet] Casset

[Histonet] Seattle-Tacoma craigslist: Manager/Senior Manager, Histology and Laboratory Resources (Bothell, WA)

seattle-tacoma craigslist / jobs / biotech/sci http://seattle.craigslist.org http://seattle.craigslist.org/see/sci/850615554.html Manager/Senior Manager, Histology and Laboratory Resources (Bothell, WA) Reply to: s

[Histonet] Microtome

Hello, We will be purchasing another microtome and I wanted to get feedback from the list.? If you wouldn't mind giving me your thoughts on the microtome you like the best.? We are not getting an automated one, not in our budget, but a simple, ever-lasting one.? We have the Leica 2135 and 2125

[Histonet] Histology schools/training in San Antonio, TX

Can someone advise me of the schools available in the San Antonio area, and what the prerequisites are? Thanks, Jackie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Oversized microscope supply source (IN USA)

We also supply many larger sizes, see www.ralamb.com Thanks, Raymond A. Lamb, Inc Jerry Helisek VP North America [EMAIL PROTECTED] 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype

[Histonet] Re: Humidity levels in the lab

>>In the summertime [the humidity in our laboratory] is at around 60-70%, which >>seems to work well. In the winter time it is less than 0 and I run a >>humidifier full out to try to achieve 20%.<< If your relative humidity is less than zero, you're probably putting too much thiotimoline in your

[Histonet] Re: Microtome micron calibration and validation

I just did an IQ, OQ, PQ on my process. The OQ on the microtome involved using a dial micrometer. Set the thickness at 5 microns. Tare or zero the dial micrometer on a point on your sample holder. Pull the micrometer point back, turn the wheel X number of times and take your new reading. It

RE: [Histonet] Humidity levels in the lab

In the summertime ours is at around 60-70%, which seems to work well. In the winter time it is less than 0 and I run a humidifier full out to try to achieve 20%. It is definitely easier in the summer. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 _

[Histonet] CE opportunity

Continuing Education Opportunity (3 CE units) Diagnostic BioSystems is sponsoring a workshop in cooperation with the California Society for Histotechnology. The workshop will be held at Mt. San Antonio College. Date: Saturday, October 18 Time: 9:30 AM to 1:00 PM Title: IHC: Basics and Beyon

[Histonet] Beta-Gal antibody

Hello, Does anyone know a reliable source for a Beta-Gal (lac-z) antibody for mouse frozen immunohistochemistry? Do you know the company and dilution? Thank you in advance, Dustin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lis

[Histonet] Microtome micron calibration and validation

Hi, I want to know ur comments and thoughts on microtome micron calibration and validation.Is there any method for doing it,please explain Thanks and Regards B.Radhika Junior Scientist Preclinical safety evaluation Dr Reddy's Discovery Research Disclaimer This message contains legally priv

[Histonet] Oversized microscope slide source

Here's a website for a company based in California, US. http://www.tedpella.com/histo_html/slides-large.htm The UK distributor is: Agar Scientific Ltd 66a Cambridge Road Stansted, Essex CM24 8DA United Kingdom Tel.: 44-1279-813519 [EMAIL PROTECTED] http://www.agarscientific.com Dodi Borsay Horo