AW: [Histonet] Gi Biopsies and the Tissue Tek VIP

2008-09-25 Thread Gudrun Lang
Laurie, do you use the biopsy-sponges with this protocol? And how many cassettes do you process at one time? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -Ursprüngliche Nachricht- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im

AW: [Histonet] Bone Marrow Biopsy Fixation

2008-09-25 Thread Gudrun Lang
Angela, we use NBF for at least 16 hours, the next day after receiving they are put in decal (mixture of formic acid and hydrochloric acid with formaldehyd) for 7-8 hours. Our biopsies are about 3-4 mm thick. After acid decalcification enzyme-stains are not possible any more and the iron-stain

[Histonet] Immunoperoxidase Control Tissue

2008-09-25 Thread JGREWE
I am interested in finding sources that sell control tissue for immunoperoxidase and special stains. Does anyone have any suggestions? Thanks Jacquelyn L. Grewe BS, HT (ASCP) Laboratory Supervisor, Anatomic Pathology Riverside Methodist Hospital 614-566-4562 or 614-566-5679

RE: [Histonet] comments/opinions on Shandon Gemini H E stainer

2008-09-25 Thread Rosa Fields
We use this stainer, I would assess it as a good purchase. It is able to accept many racks at once; easy to set up, and the tech-rite support is great. It also has a stat start, and the stainer is able to prioritize your racks for you. The racks also never come across the load and unload

[Histonet] which one first - antigen retrieval or endo peroxidase block?

2008-09-25 Thread Monfils, Paul
When doing immunoperoxidase staining on a tissue that requires antigen retrieval, which do you do first - the antigen retrieval or the endogenous peroxidase blocking? Or does it matter? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] which one first - antigen retrieval or endoperoxidase block?

2008-09-25 Thread Whitaker, Bonnie
Hi Everyone, I have always done the quench 1st if I do it manually. If it is automated, then unless you have a newer instrument with onboard retrieval, you don't have a choice but to quench after the retrieval step (and may not have a choice then depending upon the system and how open it is).

[Histonet] Job in Columbus, Ohio

2008-09-25 Thread Whitaker, Bonnie
Hi Everyone, We have a night shift opening at Ohio State University Medical Center for a senior level histotech. The ideal candidate will be HT(ASCP) or HTL(ASCP), be a motivated, self-starter, and enjoy the fast-paced environment in a large teaching hospital. OSUMC is a great place to be!

[Histonet] Need a journal atricle

2008-09-25 Thread Fidgen, Laura L.
Hello everyone, I was hoping that someone out there had access to the Applied Immunohistochemistry and Molecular Morphology journal. I need a copy of an atricle from 2007, volume 15, issue 2. Would anyone be able to help? Thank you. Laura Fidgen, HT(ASCP)cm Histology Supervisor Sacred