Laurie,
do you use the biopsy-sponges with this protocol? And how many cassettes do
you process at one time?
Gudrun Lang
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-Ursprüngliche Nachricht-
Von: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] Im
Angela,
we use NBF for at least 16 hours, the next day after receiving they are put
in decal (mixture of formic acid and hydrochloric acid with formaldehyd) for
7-8 hours. Our biopsies are about 3-4 mm thick.
After acid decalcification enzyme-stains are not possible any more and the
iron-stain
I am interested in finding sources that sell control tissue for
immunoperoxidase and special stains. Does anyone have any suggestions?
Thanks
Jacquelyn L. Grewe BS, HT (ASCP)
Laboratory Supervisor, Anatomic Pathology
Riverside Methodist Hospital
614-566-4562 or 614-566-5679
We use this stainer, I would assess it as a good purchase. It is able to
accept many racks at once; easy to set up, and the tech-rite support is great.
It also has a stat start, and the stainer is able to prioritize your racks for
you. The racks also never come across the load and unload
When doing immunoperoxidase staining on a tissue that requires antigen
retrieval, which do you do first - the antigen retrieval or the endogenous
peroxidase blocking? Or does it matter?
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Hi Everyone,
I have always done the quench 1st if I do it manually. If it is automated,
then unless you have a newer instrument with onboard retrieval, you don't
have a choice but to quench after the retrieval step (and may not have a
choice then depending upon the system and how open it is).
Hi Everyone,
We have a night shift opening at Ohio State University Medical Center for a
senior level histotech. The ideal candidate will be HT(ASCP) or HTL(ASCP),
be a motivated, self-starter, and enjoy the fast-paced environment in a large
teaching hospital.
OSUMC is a great place to be!
Hello everyone,
I was hoping that someone out there had access to the Applied
Immunohistochemistry and Molecular Morphology journal. I need a copy of an
atricle from 2007, volume 15, issue 2. Would anyone be able to help? Thank
you.
Laura Fidgen, HT(ASCP)cm
Histology Supervisor
Sacred