[Histonet] Isopropyl

Good Morning, we are interested in replacing xylene in our lab. Is anyone using graded isopropyl alcohols in there processors  instead of xylene and if so how is it working for you? Thanks.   Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ___

[Histonet] Laser Capture Microdissection Webinar

Announcing MDS Analytical Technologies next in the series of Laser Capture Microdissection and Microgenomics Webinars. Our next LCM webinar will be held on Monday, November 24, 2008 at 10AM PST or 1PM EST. The speaker will be Charmain Pietersen, PhD., Laboratory for Structural and Molecular

RE: Check out this MSDS exerpt for HistoClear RE: [Histonet]HistoClear question

And... How many of us have developed sensitivity to d-limonene? I for one have a sensitivity to it and more and more Mohs labs are forgoing fume hoods because it is considered 'safe'.   Mickie   Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC   Mohs Lab Staffing Mohs

[Histonet] Used Equipment

I am searching for a used tissue processor in good condition. Please contact me by e-mail: [EMAIL PROTECTED] Cindy DuBois Integrated Pathology Stockton, Ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

AW: [Histonet] RE: Uneven HE Staining

Are the coplin jars all equally filled? If the wash-jars are partly filled, the lower slides would be more differentiated than the upper. Gudrun -Ursprüngliche Nachricht- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Karla Arrington Gesendet: Mittwoch, 19. November 2008

RE: [Histonet] C4d question (not CD4)

Jodie, That's for CD4 not C4d Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jodie Robertson Sent: Wednesday, November 19, 2008 9:34 AM To: Richard

AW: [Histonet] Re: Telly's fixative

You have to stress the first and the third syllable. Like sun-shine-rag-gy Tell-yes-nicz-ky Gudrun -Ursprüngliche Nachricht- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Robert Richmond Gesendet: Mittwoch, 19. November 2008 02:53 An: histonet@lists.utsouthwestern.edu

RE: [Histonet] Antibody protocol

We are using the Benchmark XT. Sorry I omitted that. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jesus

RE: [Histonet] Antibody protocol

We are using the ultraView DAB from Ventana and the same HSA from Cell Marque. Here is the protocol we are using. Depar as usual; CC1 Standard; HSA incubation at 37C for 32 min; Counterstain Heme 4 min; Post Counterstain Bluing for 4 min. we have found it to be a reliable stain and very clean and

Re: [Histonet] Telly's fixative. Also Bodian.

R. Tellyesniczky published more than one fixative,nbsp;1898-190= 5. His best-known one translates into AFA or FAA: mostly ethyl alcoh= ol, with about 4% formaldehyde and about 5% acetic acid. It was = reinvented in 1937 by David Bodian (EMAnat. Rec/EM.

[Histonet] ThermoWave users

All ThermoWave Microwave Processing users Would any of you be filling to share your needle cor biopsy SOP (i.e. prostate) and the GI SOP? Thanks Roxanne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] RE: Uneven HE Staining

Sounds to me that your having a de-paraffining problem. Check your xylene or clearing agent, maybe there is water in them or they are not as clean as they should be. Are you on a daily rotation/change of staining/de-paraffin reagents? Maybe, your oven isn't hot enough to melt some paraffin

Re: [Histonet] Re: Buffer.

PIPES-buffered glutaraldehyde has been used for subsequent electron micr= oscopy since the early 1970s but it has been shown to cause artifacts- = intracellular vesicles and myelin figures -nbsp;when used for perfu= sion fixation of the brain. See FONT

[Histonet] therapeutic antibody detection in mouse xenografts?

Hi All, I am wondering if anyone has experience showing therapeutic monoclonal antibody binding in mouse xenografts (human tumor cells injected subcutaneously in SCID mice and then dose treated with therapeutic antibody injected intraperitoneally). For instance, can trastuzamab (Herceptin)

[Histonet] SPECIAL STAINER

We have used an automated special stainer for years and are now experiencing multiple problems. Therefore before we order from the same vendor I wondered what everyone else was using for silver stains such as Jones, Retic, and GMS. Just trying to evaluate if replacing instrument with newer

[Histonet] Processing problems

We had a problem with our recycler and xylene contamination so we now test each batch by adding water to a sample of recycled alcohol. If it turns cloudy we assumed that we had xylene contamination. We have lately tested commercial ethanols (no turbidity)before placing them on the processor and

Re: [Histonet] Dako

Just another FYI... The Biocare IntelliPath does the same thing, but has 4 trays of 10 and it allows you to make one of the trays?RUSH.? SO depending, on the volume?of IHC you do either of these machines would work out great. Roxanne -Original Message- From: Josie Britton

Re: [Histonet] equipment maintenance

Hi Claire Try this company, Microscope Mictorome Service Company. I use them and they are very good. Fred Siena - Tissue Techniques Pathology Labs Click to consolidate debt and lower month expenses.

RE: [Histonet] Dako

I didn't think that it had deparaffinization and retrieval on line. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Behalf Of [EMAIL PROTECTED] Sent: Wednesday, November 19, 2008 5:18 PM To: [EMAIL PROTECTED]; [EMAIL PROTECTED]; Histonet@lists.utsouthwestern.edu

Re: [Histonet] Dako

I am sorry---it is 5 trays of 10 including the RUSH tray Roxanne -Original Message- From: [EMAIL PROTECTED] To: [EMAIL PROTECTED]; [EMAIL PROTECTED]; Histonet@lists.utsouthwestern.edu Sent: Wed, 19 Nov 2008 5:17 pm Subject: Re: [Histonet] Dako Just another FYI... The Biocare

[Histonet] PSlim Slide Printer

Hello Everyone, I have been following Victor's updates on the PSlim slide printer the past couple of months. Who else is using this device out there? I have some questions about it. What kind of slides are you using? Have you had any issues with it? Thanks in advance, Tyler Liebig [EMAIL

[Histonet] RE: Histonet Digest, Vol 60, Issue 32

Histonet, anybody know where I can find Sheep Liver, we have a research person looking for a small piece or a block. Thanks in advance. Rudy [EMAIL PROTECTED] Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the 100 Best Companies to Work For in 2008

[Histonet] RE: Uneven HE Staining (Karla Arrington)

One other possiblity to consider is a thinner section at the bottom.  That could be the answer if the problem is intermittent even within the same staining rack.  If the section is thinner, the staining appears paler.   Good luck discovering the cause!

[Histonet] Uneven H E Stain

We had the same problem. The xylene had nothing to do with it and thick and thin sections was one thing that we thought might be the problem. But when I destained and restained the same slides they stained the way they were supposed to . Finally we found that we had a problem with the wash