[Histonet] Silver staing for degenerating axons

2008-12-15 Thread tf
I read the paper of Nauta with great interest! But I dont have the original pdf for the paper on Stain Technology in 1951 & 1954. Anyone can send me the pdf? I only have the improved method by Fink and Heimer in 1967 published on brain research, giving out two staining procedures. I can send yo

[Histonet] Need info on Thermo Shandon Pathcenter processors

2008-12-15 Thread Trajkovic, Dusko
Hallo Histonetters, If anyone in the Southern California area has one of these processors, can you please give me a contact person or a company that would be able to service the instrument? Thanks Dusko ___ Histonet mailing list Histonet@lists.utsouthwe

[Histonet] Embedding center and staining dishes

2008-12-15 Thread sharon . osborn
To all histotechs We have a TissueTek II Embedding console in excellent working condition that you can have by paying for the shipping charges. Remember when we were thrilled to have one of these units rather than the coffee pot or ice cream scoop to fill our blocks? We also have a co

[Histonet] pH testing

2008-12-15 Thread Hobbs, Carl
I would be very grateful if John Kiernan had the time to respond to my question. We all talk about pH but, many of us have no idea how to make sure, for eg, that we are actually measuring pH values accurately. How should we do that? I use TRIS buffer for my Immunostaining : I have a TRIS pH ele

Re: AW: [Histonet] Mucin blocking reagent for IHC

2008-12-15 Thread John Kiernan
That's a good point. The original question did not mention whether a polymer-based detection system was being used. John Kiernan Anatomy, UWO London, Canada = = = = - Original Message - From: Gudrun Lang Date: Monday, December 15, 2008 9:22 Subject: AW: [Histonet] Mucin blocking reagent

RE: [Histonet] SPECIAL STAIN PNUEMOCYSTSIS

2008-12-15 Thread Ingles Claire
We actually do that. We are lazy though, and use the water bath by our microtome(approx 43 F). After cutting is done, of course. That way you don't have anything extra to clean up. (I HOPE everyone cleans their microtome waterbaths daily!) :) We sometimes have to use the waterbath in the fume h

[Histonet] CAP and NYSDOH Regs for Staining Validation

2008-12-15 Thread McKnight, Tanisha
Hello Histonetters: Can anyone share their understanding of CAP and NYSDOH regs for validating staining and fixation procedures (not IHC)? Are the requirements for validation different for Anatomic Pathology than for Clinical Pathology? And if so, how do so of you go about performing and document

Re: [Histonet] RE: Recruiting Techs at work

2008-12-15 Thread Victoria Baker
Joelle - I am very proud of you and so would Pat. Vikki On Mon, Dec 15, 2008 at 1:29 PM, joelle weaver wrote: > > Hi Terri > I appreciated your post. I think all histotechs know that jobs are > plentiful ( at least in the current market), and most of us get lots of > postings- But, I really don

RE: [Histonet] RE: Recruiting Techs at work

2008-12-15 Thread joelle weaver
Hi Terri I appreciated your post. I think all histotechs know that jobs are plentiful ( at least in the current market), and most of us get lots of postings- But, I really don't even take notice unless there is something that is making me think and wonder if the grass is greener- this only very

Re: [Histonet] RE: Recruiting Techs at work

2008-12-15 Thread Merced Leiker
This indeed sounds like the most honest and integrous approach as well as a way to keep positions competitive. I like it. --On Monday, December 15, 2008 11:00 AM -0500 Terri Braud wrote: I agree that if you have a great work environment with competitive pay your staff will stay put, but

Re: [Histonet] RE: Recruiting Techs at work

2008-12-15 Thread Larry Woody
>>> If salaries are low, then you may have to lose a valuable tech before you >>>can make the point that salaries need to be increased.<<< 100% agreed and it's also true for trying to get some other things changed as well.    Larry A. Woody Seattle, Wa. From

[Histonet] RE: Current Regs

2008-12-15 Thread Terri Braud
Message: 1 Date: Sat, 13 Dec 2008 09:44:39 -0500 From: thisis...@aol.com Subject: [Histonet] Grosser Requirements To: histo...@lists.utsouthwestern.ed I was recently informed the CAP's grossing requirements changedI can not find anything about a change on their website. Does anyone have the cu

[Histonet] RE: Recruiting Techs at work

2008-12-15 Thread Terri Braud
I agree that if you have a great work environment with competitive pay your staff will stay put, but how many of us at the department supervisory/manager level can actually make the final decision concerning salary and pay scales for a position. I suspect that most of us, including myself, can

[Histonet] Data Entry Clerk Needed

2008-12-15 Thread Sheila Haas
We have an immediate opening for a data entry clerk with extensive medical terminology (pathology preferred) for laboratory in Lakeland, FL. Please e-mail me directly if interested.   Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Hist

AW: [Histonet] Mucin blocking reagent for IHC

2008-12-15 Thread Gudrun Lang
With the polymer-systems of IHC detection it is often seen, that in some tissueparts an unwanted background occurs. It is said, that the large polymers are trapped in OH-reach tissues like mucin and collagen. And also an additional washing step doesn't erase that. The polymers are labelled with Per

RE: [Histonet] Offended

2008-12-15 Thread Rittman, Barry R
Kimberly I think that it is a sad reflection that both management and outside companies when they hear that an employee is looking at salaries etc., assume that employee is seriously looking for a new job. This is not confined to histotech positions. I know of several instances when individuals

Re: [Histonet] Mucin blocking reagent for IHC

2008-12-15 Thread Mark Tarango
Just last week I saw DAB staining mucin for p53 (which should be nuclear staining). We were using Ventana's Iview kit which uses avidin and biotin as links. After we added the a/b block, this unwanted staining completely disappeared. I'd suggest adding a blocking step. Mark On Sun, Dec 14, 200

Re: [Histonet] Offended

2008-12-15 Thread Kimberly Tuttle
I dont think that anyone should assume that a tech is looking for another job because someone called the lab and asked for them. I get telemarketing calls from sales reps and emails and calls from job recruiters because most of them are members of this listserve, and many of our signature blocks

[Histonet] Silver staing for degenerating axons

2008-12-15 Thread tf
I read the paper of Nauta with great interest! But I dont have the original pdf for the paper on Stain Technology in 1951 & 1954. Anyone can send me the pdf? I only have the improved method by Fink and Heimer in 1967 published on brain research, giving out two staining procedures. I can send yo

Re: [Histonet] Grosser Requirements

2008-12-15 Thread Sheila Haas
I'd appreciate information on changes also. This is the first I've heard of any. Thanks!   Sheila Haas Laboratory Supervisor Micro Path Laboratories   From: "thisis...@aol.com" To: histonet@lists.utsouthwestern.edu Sent: Saturday, December 13, 2008 9:44:39 AM