Thanks very much!
i am refering to:
Doublecortin (H-280) Antibody sc-28939
http://www.scbt.com/datasheet-28939-doublecortin-h-280-antibody.html
Doublecortin (H-280): sc-28939Search | Datasheet
rabbit polyclonal IgG, 200 μg/ml
epitope corresponding to amino acids 123-402 mapping at the C-ter
I have the same post last week but did not get reply.
Actually I have a IgM primary antibody and I can just visualize the staining
with IgG 2nd antibody.
SOme IgG 2nd antibody can just applied to do so. This has been proved. But you
should test yours anyway.
2009-01-03
TF
发件人： anjan kum
Whether or not an antibody can be used for western vs IHC depends on
many factors.
Some antibodies can be used for both, some can be only used for one
vs another, and some cannot be used for either.
There are quite a few factors to consider and here are a few from the
top of my head (and I a
You can possibly use it especially if it has been used for IF. What you need
to do is to get a slide that has a known positive for the antibody, try the
dilution for WB with your standard protocol and see if it works. If is too
light or too dark you will have to titer it (which should be done in
TF and Emily,
You can use antibodies designed for Western blots. They can work. In a
western blot, a lot of times these are run under denaturing conditions (a type
of soap is used to break the secondary and tertiary structure) and so the
epitope seen is linear. That antibody for Western's wo
Is anyone using either the biogenics xmatrix or the biocare intellipath?
I am shopping and could sure use some feedback.
Thanks
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsou
If anyone replies with posting to the list, please forward it. I've always
wondered the same thing.
On Fri, Jan 2, 2009 at 8:15 AM, TF wrote:
> Hi
> Just wondering whether the antibody for WB (only WB, IP on datasheet) can
> be used for IHC use?
> If not, why?
>
> Can I just increase the concen
We do as well.
Happy 2009 Everybody!!! J
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@l
Have not tried Ventana but are very happy with the Dako Artisan.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf
Of Wendt, Karl
Sent:
Anyone out there received the new values for 2009 that start today?
Thanks
Nita
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hi,
i wanted to know if there are any difference in selecting secondary antibody
against IgM compared to secondary antibody for IgG.
as i was using CD24 which is a mouse monoclonal IgM, what kind of secondary
antibody should i select, currently i m hoping that goat anti mouse will work.
can
Hi
Just wondering whether the antibody for WB (only WB, IP on datasheet) can be
used for IHC use?
If not, why?
Can I just increase the concentration for IHC application?
I want to learn more about the underlying production processes.
Thanks!
2009-01-02
TF
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