[Histonet] Microm STP120 and 2-propanol protocol

Hello Histonetters, is there anyone with a Microm ST 120 processor? What protocol do you use? Even one with 2-propanol? Thanks for replying, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] hybridisation Oven

Hi I was wondering if anyone could provide some advice regarding hybridisation ovens. I were going to by a hybrisation oven from a company that said it came with both a carousel to rotate bottles/tubes or a rocking platform. They have since got back to me said it only comes with carousel. To buy

[Histonet] PRO Cycler BR instrument

Hi Netters, We have a Procycler by BR Instrument we have been calling the company with some questions and they refuse to send someone out to PM the recycler. We don't have major problems, but sometimes it doesn't want to read the temp probe, and we've had some issues that are 'solved' by

[Histonet] Ventana Symphony

Would like some feedback on any users out there. Any change in lab is difficult with some employees. That said, I think that it will actually be a plus in the lab---when the process changes are accepted. Any users out there have any comments? We are in evaluation period and have six more weeks

RE: [Histonet] PRO Cycler BR instrument

We have 2 and we have service agreements on them. We have no problems getting our PMs done. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] Time temp in oven prior to IHCs ?

Your drying time seems correct so you should look into other possible causes, like the infiltration or processing times. René J. --- On Tue, 1/13/09, sheila adey sheila_a...@hotmail.com wrote: From: sheila adey sheila_a...@hotmail.com Subject: [Histonet] Time temp in oven prior to IHCs ? To:

Re: [Histonet] using patient blocks for positive IHC controls

This is mostly an individual institution decision and if the block is from a case old enough as to be in the list to dispose off, there cannot be any restrictions because it is going to be disposed off anyway. René J. --- On Tue, 1/13/09, Cathy Boyd catb...@yahoo.com wrote: From: Cathy Boyd

RE: [Histonet] Microm STP120 and 2-propanol protocol

Hi Neubert, I have Microm ST 120 processor. What are you processing? Curtis D. King from that company is very good in histology contact him # 1-800-522-7270 x672 His email: ck...@rallansci.com Shakun -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Re: Refurbished Equipment

To all: I sent a recent request to the Histonet asking for people's views re: Leica's ST5020 stainer and CV5030 coverslipper. Many people replied enthusiastically about these instruments. Thank you very much for your input. I had several replies from companies who sell refurbished

[Histonet] nuetralizing DAB

Good Afternoon everyone, Is there any adverse affect if combining hydrogen peroxide and DAB when disposing? What is the proper way to dispose of stable DAB? Thank you for your reply Joselito Ferrer

[Histonet] GMA, stains, and autofluorescence

Hi, I using a procedure whereby I need the GMA resin of a thin section to fluoresce under rhodamine or UV but under other wavelengths. Currently I am staining the plastic with Eosin. Unfortunately, this causes the plastic to fluoresce under both rhodamine and FITC, something I'm trying to avoid.

[Histonet] GMA, stains, and autofluorescence

Hi, I using a procedure whereby I need the GMA resin of a thin section to fluoresce under rhodamine or UV but under other wavelengths. Currently I am staining the plastic with Eosin. Unfortunately, this causes the plastic to fluoresce under both rhodamine and FITC, something I'm trying to avoid.

[Histonet] RE: Histonet Digest, Vol 62, Issue 18

We have a Leica XL stainer with transfer station for sale. It was purchased in 2006 and only used one year. Due to consolidation of histology processing, we do not have room for this stainer. Make me an offer... Thanks, Charlotte Kopczynski, HTL (ASCP) Pathology Manager Morton Plant Mease

[Histonet] Complement C3 for mouse FFPE

Hi,   Can anyone recommend a good antibody for complement C3 on mouse FFPE tissue?   Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] murine BM monocytes

Is anyone doing F4/80 on frozen mouse bone sections? How about other monocyte markers? Would love to hear about any experiences. Thanks, Andrea -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Re: Spit and Diastase

Hi Histonetters I am one of the people in the world with deficient salivary diastase. I have been working in Histology labs for 15 years and initially had OK diastase, but over the past 4 years my saliva doesn't digest the glycogen. I do not smoke, don't have diabetes, don't chew gum

[Histonet] blocking aldehydes for staining

We are trying to use Congo Red to stain starch for confocal, but it is staining everything. The sample is condensed milk and very soft without fixation. Does anyone know of a good method to block the glutaraldehyde so that the Congo Red will only stain the starch? Bill McManus

[Histonet] staining rat tissue with monoclonal antibody?

Good afternoon. I just had someone ask me about staining rat tissue using an monoclonal (mouse) antibody. I have never done this before and so was wondering if there is anything in particular I need to keep in mind. Does mouse antibodies cause a problem when staining rat tissues? Do I have to

Re: [Histonet] blocking aldehydes for staining

Paula: Do you know of any stains that fluoresce and stain starches? On Jan 14, 2009, at 1:11 PM, Paula Pierce wrote: Congo Red is for amyloid. I suggest you use an iodine solution to stain starch. It will stain it black. From: Bill McManus bill.mcma...@usu.edu To:

[Histonet] Work flow, quality issues

I have worked in several HT labs and as expected most differ in individual technique.  Most are very good as far as work flow from grossing, processing, embedding and sectioning.  I work in a lab now where the grossing and processing of like specimens are in case order until there embedded. 

RE: [Histonet] staining rat tissue with monoclonal antibody?

Hi: We routinely perform immunohistochemistry (IHC) to detect antigens on rat tissues. A mouse mAb should be fine for use in your rat samples. You will not need a special kit, just an anti-mouse secondary Ab. One runs into trouble when they use a mouse (or rat) primary on mouse (or rat) tissue,

Re: [Histonet] staining rat tissue with monoclonal antibody?

Just make sure you use an anti-mouse IgG antibody that is not cross reactive to rat IgG. Jackson ImmunoResearch is the best place to find these reagents ... I suggest the donkey as host, it works best for me, least background. Beautiful results ... Good afternoon. I just had someone ask me

RE: [Histonet] Complement C3 for mouse FFPE

Kim: When you say 'complement C3' do you mean cleavage products of C3 after C3 activation (C3b-iC3b/C3c) or C3 iteself? C3b-iC3b/C3c are covalently attached to the surface of cells/pathogens, so I suspect this is what you would like to localize in your mouse tissue. We use an antibody in

[Histonet] Autoradiography troubleshooting urgent

Hi, I have an urgent question concerning trouble shooting of emulsion dipped histology slides. After developing emulsion dipped in situ slides stored in refrigerator for 4 weeks I found that all my slides have a cracked window appearance throughout the slide. This effect is clearly due to the

RE: [Histonet] staining rat tissue with monoclonal antibody?

We try and find a mouse anti-rat antibody if we can and run rat secondary (Dako makes one) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From:

[Histonet] Spit and Diastase

Yeah, Spitting on a slide is groody ... get a pig to do it instead hehe! Amos Message: 4 Date: Tue, 13 Jan 2009 13:16:10 -0800 (PST) From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Spit and Diastase To: histonet@lists.utsouthwestern.edu, Parker, Helayne