We would like to do immunostaining on thick FFPE sections for neuronal markers
such as tyrosine hydroxylase so that we may trace neurons contained in
non-brain tissue. Section thickness will probably be in the order of 40-50
microns, though perhaps thicker too. I have searched histonet archive
The Wright Cell Imaging Facility at University Health Network
in Toronto has compiled information on reducing autofluorescence
from the Histonet archives into a booklet.
Very useful.
http://www.uhnres.utoronto.ca/facilities/wcif/fdownload2.html
Bob
On Thu, Jan 15, 2009 at 1:36 PM, Patten, Nicol
10-15 um seems extremely thick for paraffin sections.
Can you get thinner sections? Say no thicker than 6um?
Unfortunately autofluorescence is a fact of life with FFPE sections, hence why
people normally do not choose to do immunofluorescence on FFPE sections. Are
you tethered to using immunofluo
Hi-
I am having a horrible time with autofluorescence in my human brain FFPE
tissue. I have been using Sudan Black which helps a little, but the
background is still pretty bad. The tissue is usually cut at 10-15um.
Does anyone have any other suggestions? Has anyone tried photobleaching
the tissue
You're right - "the last I heard" was about 15 years ago. It's
interesting to hear the that things we learned back then - will still kill
us today. Everything I know I learned from www.giantmicrobes.com.
Peter Carroll
Sent by: histonet-boun...@lists.utsouthwestern.edu
01/15/2009 02:2
> exposure to bleach will kill 'em - last I heard.
think about it... if that were the case, things like BSE and CJD
wouldn't be able to taint meat-processing equipment, which is generally
about as awash in bleach as anything could be, at least once per shift.
__
Beth,
We looked into several immuno stainers for over a year period and found that
the Intellipath from Bio Care beat them all out. It has the ability to run
multiple stains at one time and has a very flexible platform. You can
continuously add cases and stat cases. The support we have recei
Not only will processing NOT render it safe, but your cut, stained, and mounted
slides will still be infectious UNLESS the procedure to inactivate using formic
acid is followed before fixation and processing. If the tissue is fixed
(formalin or other common fixatives) then you would be actually
An article entitled "Safe Handling of Transmissible Spongiform Encephalopathies
Specimens in the Histopathology Laboratory" written by Konnie Zeitner was
published in the JOH June 2007..
Rosa Fields, HT (ASCP)
Gastroenterology Specialties
Histology Supervisor
4545 R Street
Lincoln, NE 68503
4
Hello Histonetters:
We are currently using Surgipath's Formula "R" paraffin. They are now charging
us a handling fee for each container. I have tried looking at different
formulas at different companies trying to find a comparable formula. It seems
that no one will list the polymer combinati
Please read the protocols at the CDC and/or the WHO website. Bleach in not
effective.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.
Viable prions have been found in >20 year old paraffin blocks.Only
extended exposure to bleach will kill 'em - last I heard. Not formalin,
not processing - nothin.
Jackie O'
Rene J Buesa
Sent by: histonet-boun...@lists.utsouthwestern.edu
01/15/2009 11:32 AM
Please respond to
rjbu...@
I'm curious if anyone out there has any feedback or comments on the new Dako
IHC Instrument, Autostainer Link 48 and/or their TPID (True Positive ID). We
are in desparate need of replacing our current DAKO instruments, and are
probably going to stick with DAKO again after looking at several ot
NO, it will not.
René J.
--- On Thu, 1/15/09, Joe Hardin wrote:
From: Joe Hardin
Subject: [Histonet] prion contaminated tissue processing
To: histonet@lists.utsouthwestern.edu
Date: Thursday, January 15, 2009, 10:29 AM
Hi All,
Does anyone know if tissue processing for paraffin embedding will r
Try phosphotungstic acid at the same concentration.
René J.
--- On Thu, 1/15/09, Kathy Lambeth wrote:
From: Kathy Lambeth
Subject: [Histonet] Wilder's Reticulum stain
To: histonet@lists.utsouthwestern.edu
Date: Thursday, January 15, 2009, 8:04 AM
--- On Thu, 1/15/09, Kathy Lambeth wrote:
Fr
Steven:
Are you just complaining to vent some sort of frustration, or can you actually
do something about it?
If venting, my sympathies go to you. What you describe could become the
nightmare of anybody with the slightest idea about histology good practices,
not to mention somebody that tries to
I made the switch to Mercedes a while back. I use their clipped corner slides
(MER7200) because they work great on our slide printer. I also use their Plus
Starfrost slides (MER7255) for our IHC. The sections stay on great, even
through antigen retrieval. Probably the best plus slide I have used
I have my techs be the only person who handles a block from facing off to
finished microtomy. They put their initials on the slide label, so if that
block needs recuts in the following days, the same tech and microtome will
be used. That way, the least amount of tissue is lost off the block fa
Joe,
I do IHC for prion diseases in animals. No, tissue processing will NOT
render prion-infected tissue safe for sectioning under normal conditions.
Incubating in formic acid won't even totally inactivate the abnormal
protein. We have to use a dedicated microtome in a dedicated room, wear
Joe,
You might want to get a hold of somebody over at the WVDL. I know that when I
worked there, we wore gloves when sectioning any blocks that may have come from
deer with CWD ie were potentially contaminated with prion.
Tom Pier
>>> Joe Hardin 01/15/09 9:32 AM >>>
Hi All,
Does anyone know i
We use their cheaper ones - CAS 9308 W - and they work just fine. j
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jamie E
Erickson
Sent: Thursday, January 15, 2009 10:28 AM
To: histonet histonet
Subject: [
What is the gain??? I am confused by their methods. I also face(rough cut)
and section on the same microtome. I noticed over the years that cuts on the
same microtome and block, one tech can get a thin section just by the way
they turn the flywheel. A heavy handed cutter can make the section thicke
It will not. Check out the CDC and/or the WHO guidelines.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf O
Hi All,
Does anyone know if tissue processing for paraffin embedding will render
prion infected tissue safe for sectioning?
___
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Geisinger Medical Center in Danville, PA is searching for two
Histotechnologists. We have a dayshift 0.6 tech position and a nightshift 8P-4A
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information about our Health System. Contact me for more information at
570-21
Hi All,
I was wondering if anyone out there has used Mercedes
medical starfrost adhesive slides and how they hold up in IHC.
MER 7255 - Slides, adhesive, 90, Starfrost, 10 gross - $235.
I am thinking about changing to these slides from our VWR superfrost plus
slides to save money
Hi Everyone :)
Does anyone do or know of antibodies for immuno staining of muscle for
fiber typing?? If you do catalog numbers and company that you get the
antibodies from would be greatly appreciated and also your procedure
would also be nice :)
Neuropath Nancy @ RIH
--- On Thu, 1/15/09, Kathy Lambeth wrote:
From: Kathy Lambeth
Subject: Wilder's Reticulum stain
To: histo...@list.utsouthwestern.edu
Date: Thursday, January 15, 2009, 6:25 AM
I just relocated to a new lab and they use Wilder's Retic stain. They have
been unable to get uranium nitrate.
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