After 30 years in histology I say go with VIP for reliable processing. (never
had one fail or damage tissue)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Farnham, Lori
Sent: Wednesday, March 04, 2009
I was wondering if anybody might have an idea with the following
problem we are experiencing: we want to stain for blood vessels in
sections of mouse brain. Our experimental tissues have been fixed
overnight in 4% paraformaldehyde and have been sitting in PBS since.
We have tried
CD56 (NCAM1) and CD16 (Fc gamma receptor III) are used to define human
NK cell populations. Subsets of NK cells are distinguished from one
another based on 'bright' or 'dim' expression of these two markers.
Refer to Blood Rev. 20(3):123-37 (2006) for further information.
---mtp
Michele T.
We use it for fatty samples (we do lots of breast) and yes, it does work
well.
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-Original Message-
From:
hi, CD31 works great
in my section alpha-SMA also works
another way is to perfuse the brain with BSA-rhodamine.
you will get the fluorescence without the need of staining.
2009-03-05
TF
发件人： iskaliora
发送时间： 2009-03-05 18:49:11
收件人： histonet
抄送： ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ
主题： [Histonet]
Hi Patsy and Anjan Kumar,
VectaMount is not aqueous, but an organic mountant too. It does not contain
alcohol or xylene, in fact it hardly smells. It certainly does not mix with
water. We use this mountant by washing the slide with distilled water and then
let it dry completely at a 50C
Hartmann's fixative:
Hello All,
I first became aware of this fixative in when still doing my internship from
histology school at Compunet Clinical laboratory in Dayton, OH. I was asked
by my technical director, Alice L. Trent to make it up from scratch, even
through it is called Hartmann's
Alice says you're welcome!
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Valantou Grover, HT/HTL(ASCP), PA, MBA
Biosciences Product Line Manager
Polysciences, Inc.
400 Valley Road
Warrington, PA 18976
Fax: 1-800-323-3291
Phone number: 1-800-523-2575 X7418
Direct:1-215-488-7418
Cell phone: 1-215-409-8327
-Original Message-
From: Valantou Grover
Does anyone know of any companies producing charged full mount slides? I
have a pathologist that wants immunos done on full mount prostates and
the tissue keeps falling off. I have looked, but cannot find any charged
full mount slides. Any help would be appreciated!!
Kenneth G Metzger
We also addes 4-5 drops of Tween 20 in waterbath.
Human eye's sections have not any wrinlkes.
Maxim Peshkov,
Russia,
Taganrog.
--- On Wed, 3/4/09, Perry, Margaret
From: Perry, Margaret margaret.pe...@sdstate.edu
Subject: [Histonet] problem eyes
To: histonet@lists.utsouthwestern.edu
You will have to silanize your slides.
René J.
--- On Thu, 3/5/09, Metzger, Kenneth kenneth.metz...@aruplab.com wrote:
From: Metzger, Kenneth kenneth.metz...@aruplab.com
Subject: [Histonet] Full Mount Immunos
To: histonet@lists.utsouthwestern.edu
Date: Thursday, March 5, 2009, 10:07 AM
Does
I believe that Brain Research Labs has large + slides.
Helen
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth
Sent: Thursday, March 05, 2009 10:07 AM
To: histonet@lists.utsouthwestern.edu
Can anyone recommend a good aqueous mounting media for coverslipping Oil
Red O stained slides? We are currently using Dako's Faramount media, but
have been having problems with excessive air bubbles; especially, it
seems, in the tissues containing a lot of fat. I recently heard that
Dako's
Anyone know the Abcam CD31 on rat brain?
http://www.abcam.com/CD31-antibody-ab28364.html
Or suggest another one?
2009-03-06
TF
发件人： Colleen Forster
发送时间： 2009-03-06 00:57:29
收件人： tifei
抄送： iskaliora; histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ
主题： Re: [Histonet] staining brain vessels
You have
We run a third shift with 2 people and they do all the biopsies and stat
specimens. This works great if you can keep the shift staffed. I have had to
replace 5 people in 2 years. I am currently looking into getting a rapid
processor so I do the biopsies during the day and totally eliminate my
We are looking at the VIP 6 and the Leica ASP300, I was wondering if any one
has any experience with the VIP6.
Nathaniel
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Weihua,
I prefer iron gallein elastin stain because it is = very simple to
make and use and gives good contrast compared with some of t= he other
techniques. The recipes and protocol can be found in Humanson's An imal
Tissue Techniques (in addition to many other
We are looking for a supervisory level Histologist with experience in IHC to
complement our laboratory staff. If you are interested please forward a rsume
to
Christine Sullivan
Business manager
LifePath Partners, LLC
299 Carew St.
Springfield, MA 01104
413-748-9779
christine.sulli...@sphs.com
The rabbit pAb antibody from Molecular Probes invitrogen works great in frozen
and fixed frozen so it might work in paraffin. Worth a shot
Andrea
--Original Message--
From: Michele Wich
Sender: histonet-boun...@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Sent: Mar
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