[Histonet] Permanent Job Openings with Medical Diagnostic Company in New Jersey

2009-03-16 Thread Frederick, David
Good morning, I am hiring for several permanent positions with a growing medical diagnostic company here in Central New Jersey. My client has several IMMEDIATE openings for Histology Technicians who have experience with Gram Staining, Section Cutting and Paraffin Embedding. Hands-on

[Histonet] decolorizing MMA

2009-03-16 Thread Molinari, Betsy
Good morning, Is it possible to decolorize MMA slides? Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ___ Histonet mailing

RE: [Histonet] decolorizing MMA

2009-03-16 Thread Patsy Ruegg
Do you mean deplasticize, as in remove the resin? If that is what you mean, yes, you can remove mma with xylene, you cannot remove GMA. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org

RE: [Histonet] decolorizing MMA

2009-03-16 Thread Swain, Frances L
If you want to remove the MMA you can also use 2-Methoxyethyl Acetate or Acetone. If you want to decolorize like a hematoxylin, yes you can do that also. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton

[Histonet] grossing

2009-03-16 Thread godsgalnow
I hate to beat a dead horse.but how do you handle non-certified grossing staff.? They all meet the CLIA guidelines, but are they allowed to gross in the evening without the presence of a technical supervisor? And how do you handle salaries for those that process and those that

Re: [Histonet] grossing

2009-03-16 Thread Histonet Alias
Define presence? If they are able to have access a technical supervisor or a Pathologist if they have a question then this may just cover it. We have a Pathologist on call that can be in within minutes if there are any questions. On Mon, Mar 16, 2009 at 10:55 AM, godsgal...@aol.com wrote: I

[Histonet] Antibody advice

2009-03-16 Thread Mark Elliott
Good morning everyone We are starting a rather large project and I would like to get some input as to the best version of antibodies to the following antigens-what are people using for the following and where do you get them from. This will be either frozen sections or formalin fixed paraffin

Re: [Histonet] grossing

2009-03-16 Thread Rene J Buesa
From my point of view, shared by all AP and many managers, the answer is NO, they cannot, and should not. Histology samples are too precious to be handled by somebody without the proper training. René J. --- On Mon, 3/16/09, godsgal...@aol.com godsgal...@aol.com wrote: From: godsgal...@aol.com

Re: [Histonet] grossing

2009-03-16 Thread godsgalnow
René, my friend This is my opinion as well and I have stated so, but is there any documentation? Roxanne -Original Message- From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; godsgal...@aol.com Sent: Mon, 16 Mar 2009 11:24 am Subject: Re: [Histonet]

[Histonet] TweakR

2009-03-16 Thread Melissa Mazan
Hi all, I'm trying to use Tweak R (Fn14) on mouse lung. I've got a rabbit polyclonal from abcam that supposedly is validated for IHC-paraffin - have tried it with and without HIER (citrate) and with and without detergents - was worried that as its a transmembrane protein maybe I was washing

RE: [Histonet] IHC stainer

2009-03-16 Thread Bower, Jennifer
I am in a research lab and we are currently testing automated IHC machines. A couple months ago we had the Bond Max from Leica. The machine gave me nothing but problems. They said it was because it was a demo unit and the real one would not do that (who knows). The interesting thing, though, was

[Histonet] Looking for Patsy Ruegg's e-mail address

2009-03-16 Thread Perry, Margaret
Patsy I have a couple of questions for you but have the wrong address. Margaret Perry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Histotechnologist Position in Boston-Direct Hire

2009-03-16 Thread Alyssa Peterson
Hello Histonetters! I hope everyone had a great weekend. I wanted to follow up in reference to a full time, direct hire position for a histotechnologist in Boston. My company offers a referral bonus up to $1000 paid to you if your referral is hired. Forward this to anyone you feel fit. If

Re: [Histonet] grossing

2009-03-16 Thread godsgalnow
Actually, as long as they meet the standards for High Complexity testing they can gross...according to CAP you have to have no knowledge of anatomy to process. Roxanne -Original Message- From: Histonet Alias histonetal...@gmail.com To: godsgal...@aol.com Cc:

RE: [Histonet] grossing

2009-03-16 Thread dcojita
According to CLIA, the employee who grosses must also possess a state license when working in a state that requires licensure, such as Florida. Keep in mind that CAP rules do not apply for those laboratories that are not CAP accredited. Exert from CLIA - In order to qualify as high

[Histonet] BrdU immunostain on rat brain section

2009-03-16 Thread Amy Lee
Hello, I was asked to do BrdU immunostain on rat brain tissue. Does anyone have experience of it? 1.Do you prefer frozen or paraffin? 2.Which antibody you recommend? 3. If  35um frozen is requied, what thickness you will use if you want go for paraffin?     Thanks, Amy

[Histonet] Fix Sediment Method

2009-03-16 Thread Knutson, Deanne
Fellow Histonetters - I am looking for feedback on the alternative methods to prepare Non GYN cellblocks. We currently use the Fix Sediment Method where you spin the fluid to achieve a button, pour off the supernatant, and pour formalin onto the button to harden it. What do others do? Does

Re: [Histonet] BrdU immunostain on rat brain section

2009-03-16 Thread TF
hi, we worked a lot on this. I prefer to use 40 um frozen sections. Abcam anti-BrdU (rat sourced) is the best ever I know. It does not have high background with rat IgG. Some other mouse-/sheep- sourced BrdU are available. 2009-03-17 TF 发件人: Amy Lee 发送时间: 2009-03-17 05:02:28 收件人: